APPLICATIONS
Electrophysiology
Electrophysiology requires slicing of live soft tissues. The quality of your experiment depends on the quality of the tissue slice. Your daily productivity also depends on the quality of the tissue slice. The viability of the brain slice, especially the healthy neurons in the top surface layers are crucial to the success of brain slice patch clamp electrophysiology. The Compresstome™ semi-automated  VF-200-0z  and fully automated  VF-300-0z  produce the highest quality brain slices for these researches.
Electrophysiology at The Allen Institute
One of the things that we are keen to do at Precisionary Instruments to help push the boundaries of scientific research.  That's why it's always cool to see how scientists have modified and enhanced the Compresstome.  At the Allen Institute, their "open science" concept puts neuroscientists together with system engineers to come up with some extremely cool ideas, and conjure them into existence.

We are proud that the Compresstome can be part of this (at 1:34-1:50 and 3:10), helping the scientists work with both mouse brains as well as human brain tissues.

Why Compresstome?

Doubling the slice viability:  The patented Compression technology and our auto zero Z feature stabilizes both the balde and the tissue, giving you many good neurons on the top surface that can be chosen for experiment. Greatly increases your productivity.
 
Best for older animal and more sensitive brain areas:  Vulnerable tissues of older animals and more sensitive nuclei of the brain can still be sliced with the Compresstome™ VF-200 and VF-300, greatly expanding the range of experiments that you can do.
 
Smoother and cleaner cutting surface:  The wavy chatter marks indicate the trails of the oscillating blade of the slicer. In the VF-200 and VF-300 microtome, the vibration mark is eliminated from the slice surface.
 
High quality images: Images of slices cut with the Compresstome™ VF-200 and VF-300 are sharper ,with fewer scratches and distortion.
 
Reduce thermal shock:  Slicing can be performed in warm temperature (32 degrees C) as well as 4 degrees C. The ability to slice in warm temperature eliminates drastic temperature fluctuation, reducing thermal shock to the tissues.
 
Quick recovery: The ability to slice in warm temperature allows recovery in less than 20 minutes.
 
Consistent slice thickness:  The unique specimen embedding, holding and clamping mechanisms minimizes specimen movement in all three dimensions during slicing. Slice thickness varies less than 5 percent of the slice thickness (250 µm), compared to 10 to 20 percent in classic slicers.
 
Versatile:  Need to do some immunohistochemistry work as well? The VF-300 is also great at slicing fixed tissues, saving your money and lab space.
 
Fast cutting:  For vibrating blade tissue slicers, it takes up to 30 seconds to cut a slice. The VF-200 and VF-300 takes less than 5 second to cut a slice.
 
Simple:
The operation of the VF-200 and VF-300 are simpler than that of other vibrating slicers. Close monitoring is unnecessary. You can learn it by watching a couple of Youtube videos.
  1. "For the past several months, I have been using the Precisionary Instruments Inc VF-200 to cut 300 um prefrontal cortical slices for electrophysiological recordings. As a result, I have been consistently cutting exceptional quality brain slices and have succeeded in obtaining stable whole-cell patch recordings from numerous neurons. Because I am using transgenic mice that have fluorescent reporters, it is of great importance to achieve maximal cell viability and minimal cell death. When I use the VF-200, I have significantly greater number of surface neurons than those slices cut from a regular Vibratome™. In addition the slice surface is much more even, which allows for better imaging. I am thoroughly impressed with the VF-200 and would not hesitate recommending this quality product to fellow electrophysiologists ."
    Newton Woo, PhD NICHD/NI
  2. We obtained a VF-300 Compresstome™ about 5 months ago to use alongside a microtome that we had exclusively used for at least 5 years prior. The VF-300 is more consistent in generating uniform slices and leaves the structure of the tissue/cells more intact than the method we had been using. We have been able to extend the amount of time over which we can cut lung slices, and allows preservation of the architecture of the slice and greater viability of the tissue.
    Dr. Cynthia Kozial-White, University of Pennsylvania
  3. With the Compresstome™ I was able to prepare high quality slices with excellent preservation near the slice surface. The main advantages of this machine are the rapid speed of slicing and the manual stabilization afforded by the agarose embedding. I am able to prepare uniform slices in both the coronal and horizontal planes, and I routinely complete transcardial perfusion, brain removal, and slicing all within less than 10 minutes, which is half the time needed with the Vibratome model. By limiting the time required for slicing the brain tissue is able to recover more easily without significant anoxia, and my slices stay viable for up to 8 hours. I have successfully been able to do targeted whole-cell recordings from fluorescently labeled neurons in slices prepared from >1 year old mice, which is not generally possible with conventional slicers and methods.
    Dr. Jonathan Ting, Allen Institute
  4. We found the Compresstome produces a less apparent damage to the cells of the airways and arterioles as well as to the delicate alveolar parenchyma. Using the Compresstome we are able to obtain more reproducible and successful experiments.
    Dr. Perez-Zoghbi, Texas Tech University
  5. We spent six months trying to cut our tissue (avian olfactory) with a Vibratome..... but without success. It took just 10 minutes with the Compresstome, and the sections are beautiful. It's the best investment my lab has made.
    Dr. David Keays, Institute of Molecular Pathology Vienna
  6. At first, I was skeptical about the VF-200, but the results were astonishing. I have been using a Vibroslice ™ (Campden Instruments, Leicester , UK) for over 13 years, and I would never get so many living cells in one field as with the VF-200. This is also true and especially impressive for tissue of older animals. The oldest rats we used were 31 days of age. The cell quality was very good and the field was very clean. The VF-200 has greatly improved our productivity. Many thanks to Precisionary Instruments Inc. for creating such a wonderful instrument, and for their significant contribution to the neuro-science field.
    Dr. Jiang-Hong Ye, University of Medicine and Dentistry of New Jersey