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Immunohistochemistry

Our Compresstome™ is specially designed for cutting fixed tissue slices for floating section immunohistochemistry. Because of its many advantages, it is the machine of choice for floating section studies. The Compresstome™ can cut slices as thin as 3 microns, equivalent in thickness to 2 microns of dehydrated paraffin-embeded slices.
The VF-300-0Z with the automatic zero Z feature is the recommended Compresstome for this application.

 

-For tissue samples larger than 15.5 mm in diameter, we recommend the VF-700-0Z.
-For tissue samples larger than 22 mm in diameter, we recommend the VF-800.
-For tissue samples larger than 95 mm in diameter, we recommend the VF-900.

Advantages over the Cryostat
Simpler Protocol: There are only three steps for the Compresstome™ protocol –tissue fixation, tissue slicing, and free floating staining. No over night sucrose incubation, no OCT compound embedding, no liquid nitrogen freezing. This saves you time and money.  It also makes the experiment much more repeatable.
Stronger signals: The Compresstome™ eliminates the freeze-thaw step(s) that can lead to degradation in proteins and their antigenicities. This reduces false negative results for low level signals.
Better histology images:  There is no ice crystal formation with the Compresstome™ slice. Cellular structure and histology image are better preserved. Further EM study is possible.
Wider cutting window: The cyrostat can slice tissues between 4 to 40 microns. For experiments requiring thicker slices, the cryostat slices cracks easily. The Compresstome™ can cut slice thickness from 8 micron and up, with no upper thickness limit.
Convenient:  The footprint of the Compresstome™ is very small. It can be easily moved around from counter to counter.
Affordable:  The total cost for the Compresstome™ is only a small fraction of that of a cryostat. The savings on material cost and your time is even bigger.

Advantages over a traditional Vibratome

Easy to use:  The operation of the Compresstome™ is simpler than that of other vibrating slicers. Close monitoring is unnecessary. You can learn how to use it by watching 2-3 short 3 minute videos online.
Thinner slices:  With traditional vibrating slicers, you start seeing slices shredding around 20 microns.  With the Compresstome™, you can consistently section slices at 3 microns.
Smoother slices:  The wavy chatter marks or vibration marks indicate the trails of the oscillating blade of the slicer. In the Compresstome™, the vibration mark is completely eliminated from the slice surface by our patented Compression technology.
High quality images:  Images of slices cut with the Compresstome™ are sharper ,with fewer scratches and distortion.
Versatile:  Need to do some electrophysiology work as well? The VF-300 Zero Z is also great at slicing acute tissues, saving your money and lab space.
Consistent slice thickness:  The unique specimen embedding, holding and clamping mechanisms minimizes specimen movement in all three dimensions during slicing. Consistent slice thickness can be achieved. Slice thickness varies less than 5 percent of the slice thickness (250 µm), compared to 10 to 20 percent in classic slicers.
Fast cutting:  For vibrating blade tissue slicers, it takes up to 30 seconds to cut a slice. The Compresstome™ takes less than 2 seconds to cut a slice.