Applications
Immunohistochemistry
Our Compresstome™ is specially designed for cutting fixed tissue slices for floating section immunohistochemistry. Because of its many advantages, it is the machine of choice for floating section studies. The Compresstome™ can cut slices as thin as 3 microns, equivalent in thickness to 2 microns of dehydrated paraffin-embeded slices.
 
The VF-300-0Z with the automatic zero Z feature is the recommended Compresstome for this application.
 
  • For tissue samples larger than 15.5 mm in diameter, we recommend the VF-700-0Z .
  • For tissue samples larger than 22 mm in diameter, we recommend the VF-800 .
  • For tissue samples larger than 95 mm in diameter, we recommend the VF-900 .
Advantages over the Cryostat
Simpler Protocol: There are only three steps for the Compresstome™ protocol --tissue fixation, tissue slicing, and free floating staining. No over night sucrose incubation, no OCT compound embedding, no liquid nitrogen freezing. This saves you time and money.  It also makes the experiment much more repeatable.
 
Stronger signals: The Compresstome™ eliminates the freeze-thaw step(s) that can lead to degradation in proteins and their antigenicities. This reduces false negative results for low level signals.
 
Better histology images:  There is no ice crystal formation with the Compresstome™ slice. Cellular structure and histology image are better preserved. Further EM study is possible.
 
Wider cutting window: The cyrostat can slice tissues between 4 to 40 microns. For experiments requiring thicker slices, the cryostat slices cracks easily. The Compresstome™ can cut slice thickness from 8 micron and up, with no upper thickness limit.
 
Convenient:  The footprint of the Compresstome™ is very small. It can be easily moved around from counter to counter.
 
Affordable:  The total cost for the Compresstome™ is only a small fraction of that of a cryostat. The savings on material cost and your time is even bigger.
Advantages over a traditional Vibratome
Easy to use:  The operation of the Compresstome™ is simpler than that of other vibrating slicers. Close monitoring is unnecessary. You can learn how to use it by watching 2-3 short 3 minute videos online.
 
Thinner slices:  With traditional vibrating slicers, you start seeing slices shredding around 20 microns.  With the Compresstome™, you can consistently section slices at 3 microns.
 
Smoother slices:  The wavy chatter marks or vibration marks indicate the trails of the oscillating blade of the slicer. In the Compresstome™, the vibration mark is completely eliminated from the slice surface by our patented Compression technology.
 
High quality images:  Images of slices cut with the Compresstome™ are sharper ,with fewer scratches and distortion.

Versatile:  Need to do some electrophysiology work as well? The VF-300 Zero Z is also great at slicing acute tissues, saving your money and lab space.
Consistent slice thickness:  The unique specimen embedding, holding and clamping mechanisms minimizes specimen movement in all three dimensions during slicing. Consistent slice thickness can be achieved. Slice thickness varies less than 5 percent of the slice thickness (250 µm), compared to 10 to 20 percent in classic slicers.
 
Fast cutting:  For vibrating blade tissue slicers, it takes up to 30 seconds to cut a slice. The Compresstome™ takes less than 2 seconds to cut a slice.
 
  1. "For the past several months, I have been using the Precisionary Instruments Inc VF-200 to cut 300 um prefrontal cortical slices for electrophysiological recordings. As a result, I have been consistently cutting exceptional quality brain slices and have succeeded in obtaining stable whole-cell patch recordings from numerous neurons. Because I am using transgenic mice that have fluorescent reporters, it is of great importance to achieve maximal cell viability and minimal cell death. When I use the VF-200, I have significantly greater number of surface neurons than those slices cut from a regular Vibratome™. In addition the slice surface is much more even, which allows for better imaging. I am thoroughly impressed with the VF-200 and would not hesitate recommending this quality product to fellow electrophysiologists ."
    Newton Woo, PhD NICHD/NI
  2. We obtained a VF-300 Compresstome™ about 5 months ago to use alongside a microtome that we had exclusively used for at least 5 years prior. The VF-300 is more consistent in generating uniform slices and leaves the structure of the tissue/cells more intact than the method we had been using. We have been able to extend the amount of time over which we can cut lung slices, and allows preservation of the architecture of the slice and greater viability of the tissue.
    Dr. Cynthia Kozial-White, University of Pennsylvania
  3. With the Compresstome™ I was able to prepare high quality slices with excellent preservation near the slice surface. The main advantages of this machine are the rapid speed of slicing and the manual stabilization afforded by the agarose embedding. I am able to prepare uniform slices in both the coronal and horizontal planes, and I routinely complete transcardial perfusion, brain removal, and slicing all within less than 10 minutes, which is half the time needed with the Vibratome model. By limiting the time required for slicing the brain tissue is able to recover more easily without significant anoxia, and my slices stay viable for up to 8 hours. I have successfully been able to do targeted whole-cell recordings from fluorescently labeled neurons in slices prepared from >1 year old mice, which is not generally possible with conventional slicers and methods.
    Dr. Jonathan Ting, Allen Institute
  4. We found the Compresstome produces a less apparent damage to the cells of the airways and arterioles as well as to the delicate alveolar parenchyma. Using the Compresstome we are able to obtain more reproducible and successful experiments.
    Dr. Perez-Zoghbi, Texas Tech University
  5. We spent six months trying to cut our tissue (avian olfactory) with a Vibratome..... but without success. It took just 10 minutes with the Compresstome, and the sections are beautiful. It's the best investment my lab has made.
    Dr. David Keays, Institute of Molecular Pathology Vienna
  6. At first, I was skeptical about the VF-200, but the results were astonishing. I have been using a Vibroslice ™ (Campden Instruments, Leicester , UK) for over 13 years, and I would never get so many living cells in one field as with the VF-200. This is also true and especially impressive for tissue of older animals. The oldest rats we used were 31 days of age. The cell quality was very good and the field was very clean. The VF-200 has greatly improved our productivity. Many thanks to Precisionary Instruments Inc. for creating such a wonderful instrument, and for their significant contribution to the neuro-science field.
    Dr. Jiang-Hong Ye, University of Medicine and Dentistry of New Jersey