Enabling Research on Elderly Diseases
Many disease models, genetic defects and geriatric diseases do not show symptoms until adult or senior age. In neuroscience researches, one of the limitations for acute brain slice model is that it is very difficult to obtain healthy slices from older animals. Over 90% of the brain slice studies were carried out on animals younger than 40 days (mice or rats). There usually was a time gap between the cell physiology data and the onset of the disease symptoms that makes data correlation or interpretation difficult.
Recently, there is a substantial progress in the method for preparing adult brain slices. By using Compresstome™ microtome and a unique sectioning protocol, Dr. Ting at the Allen Institute can routinely obtain healthy adult and senior adult brain slices for patch clamp recording. This allows much more accurate study of elderly diseases.  You can discover the procedures and methodologies of this approach at
The Compresstome™ semi-automated  VF-200-0z  and fully automated  VF-300-0z  produce the highest quality brain slices for these researches.
18 month old mouse - striatal cholinergic neuron 
  1. "For the past several months, I have been using the Precisionary Instruments Inc VF-200 to cut 300 um prefrontal cortical slices for electrophysiological recordings. As a result, I have been consistently cutting exceptional quality brain slices and have succeeded in obtaining stable whole-cell patch recordings from numerous neurons. Because I am using transgenic mice that have fluorescent reporters, it is of great importance to achieve maximal cell viability and minimal cell death. When I use the VF-200, I have significantly greater number of surface neurons than those slices cut from a regular Vibratome™. In addition the slice surface is much more even, which allows for better imaging. I am thoroughly impressed with the VF-200 and would not hesitate recommending this quality product to fellow electrophysiologists ."
    Newton Woo, PhD NICHD/NI
  2. We obtained a VF-300 Compresstome™ about 5 months ago to use alongside a microtome that we had exclusively used for at least 5 years prior. The VF-300 is more consistent in generating uniform slices and leaves the structure of the tissue/cells more intact than the method we had been using. We have been able to extend the amount of time over which we can cut lung slices, and allows preservation of the architecture of the slice and greater viability of the tissue.
    Dr. Cynthia Kozial-White, University of Pennsylvania
  3. With the Compresstome™ I was able to prepare high quality slices with excellent preservation near the slice surface. The main advantages of this machine are the rapid speed of slicing and the manual stabilization afforded by the agarose embedding. I am able to prepare uniform slices in both the coronal and horizontal planes, and I routinely complete transcardial perfusion, brain removal, and slicing all within less than 10 minutes, which is half the time needed with the Vibratome model. By limiting the time required for slicing the brain tissue is able to recover more easily without significant anoxia, and my slices stay viable for up to 8 hours. I have successfully been able to do targeted whole-cell recordings from fluorescently labeled neurons in slices prepared from >1 year old mice, which is not generally possible with conventional slicers and methods.
    Dr. Jonathan Ting, Allen Institute
  4. We found the Compresstome produces a less apparent damage to the cells of the airways and arterioles as well as to the delicate alveolar parenchyma. Using the Compresstome we are able to obtain more reproducible and successful experiments.
    Dr. Perez-Zoghbi, Texas Tech University
  5. We spent six months trying to cut our tissue (avian olfactory) with a Vibratome..... but without success. It took just 10 minutes with the Compresstome, and the sections are beautiful. It's the best investment my lab has made.
    Dr. David Keays, Institute of Molecular Pathology Vienna
  6. At first, I was skeptical about the VF-200, but the results were astonishing. I have been using a Vibroslice ™ (Campden Instruments, Leicester , UK) for over 13 years, and I would never get so many living cells in one field as with the VF-200. This is also true and especially impressive for tissue of older animals. The oldest rats we used were 31 days of age. The cell quality was very good and the field was very clean. The VF-200 has greatly improved our productivity. Many thanks to Precisionary Instruments Inc. for creating such a wonderful instrument, and for their significant contribution to the neuro-science field.
    Dr. Jiang-Hong Ye, University of Medicine and Dentistry of New Jersey