APPLICATIONS
Precision-Cut Tissue Slices
Precision-cut tissue slices allows researchers to observe and assess organ functions under cell culture conditions. The method allows the study of metabolism for almost any organ system.

Applicable organ systems include:

- Respiratory system (Precision-cut lung slices)
- Hepatology system (Precisionary-cut liver slices)
- Gastrointestinal system (Precision-cut gut slices)
- Renal system (Precisionary-cut kidney slices)

Precision-cut slices require healthy, viable tissue slices in order for metabolism studies to yield accurate results.
 
Slicing is the first and most important step of this procedure.  The ability to cut the perfect tissue slice to minimize cellular damage is cruial for subsequent experiments.
 
The traditional method of using a Krumdieck tissue slicer introduces unwanted tissue stretching and causes damage to the cells on these slices.  With our patented compression technology, the Compresstome™ can provide healthier slices with more viable cells for your metabolism, pharmacology, and toxicology experiments.

Exactly how is the Compresstome better than a Krumdieck slicer?
  • Yields healthier slices for metabolism studies
  • Gives more viable cells for experiments
  • Allows you to section a "core" of tissue
  • Applications include pharmacolog & toxicology studies
  • You can control the slice thickness!
  • Get consistent slice thickness with every cut
 
The Compresstome™ semi-automated  VF-200-0z  and the fully automated VF-300-0z produce the highest quality precision-cut tissues slices.

Need the Protocol?   We have that, too!

 
  1. "For the past several months, I have been using the Precisionary Instruments Inc VF-200 to cut 300 um prefrontal cortical slices for electrophysiological recordings. As a result, I have been consistently cutting exceptional quality brain slices and have succeeded in obtaining stable whole-cell patch recordings from numerous neurons. Because I am using transgenic mice that have fluorescent reporters, it is of great importance to achieve maximal cell viability and minimal cell death. When I use the VF-200, I have significantly greater number of surface neurons than those slices cut from a regular Vibratome™. In addition the slice surface is much more even, which allows for better imaging. I am thoroughly impressed with the VF-200 and would not hesitate recommending this quality product to fellow electrophysiologists ."
    Newton Woo, PhD NICHD/NI
  2. We obtained a VF-300 Compresstome™ about 5 months ago to use alongside a microtome that we had exclusively used for at least 5 years prior. The VF-300 is more consistent in generating uniform slices and leaves the structure of the tissue/cells more intact than the method we had been using. We have been able to extend the amount of time over which we can cut lung slices, and allows preservation of the architecture of the slice and greater viability of the tissue.
    Dr. Cynthia Kozial-White, University of Pennsylvania
  3. With the Compresstome™ I was able to prepare high quality slices with excellent preservation near the slice surface. The main advantages of this machine are the rapid speed of slicing and the manual stabilization afforded by the agarose embedding. I am able to prepare uniform slices in both the coronal and horizontal planes, and I routinely complete transcardial perfusion, brain removal, and slicing all within less than 10 minutes, which is half the time needed with the Vibratome model. By limiting the time required for slicing the brain tissue is able to recover more easily without significant anoxia, and my slices stay viable for up to 8 hours. I have successfully been able to do targeted whole-cell recordings from fluorescently labeled neurons in slices prepared from >1 year old mice, which is not generally possible with conventional slicers and methods.
    Dr. Jonathan Ting, Allen Institute
  4. We found the Compresstome produces a less apparent damage to the cells of the airways and arterioles as well as to the delicate alveolar parenchyma. Using the Compresstome we are able to obtain more reproducible and successful experiments.
    Dr. Perez-Zoghbi, Texas Tech University
  5. We spent six months trying to cut our tissue (avian olfactory) with a Vibratome..... but without success. It took just 10 minutes with the Compresstome, and the sections are beautiful. It's the best investment my lab has made.
    Dr. David Keays, Institute of Molecular Pathology Vienna
  6. At first, I was skeptical about the VF-200, but the results were astonishing. I have been using a Vibroslice ™ (Campden Instruments, Leicester , UK) for over 13 years, and I would never get so many living cells in one field as with the VF-200. This is also true and especially impressive for tissue of older animals. The oldest rats we used were 31 days of age. The cell quality was very good and the field was very clean. The VF-200 has greatly improved our productivity. Many thanks to Precisionary Instruments Inc. for creating such a wonderful instrument, and for their significant contribution to the neuro-science field.
    Dr. Jiang-Hong Ye, University of Medicine and Dentistry of New Jersey