A: The VF-200 is the semi-automatic model with a slice thickness resolution of 10 microns. You have to manually advance the tissue through the tube with the micrometer. Thus, it is suitable for slicing live tissues for electrophysiology and tissue culture where the thickness requirement is 150 micron and up.
The VF-300 is a fully automatic model with a slice thickness resolution of 1 micron. It has a digital control that advances the tissue with extreme accuracy. The VF-300 also has a continuous slicing mode in which you can set the per-slice thickness and hit the start button. The microtome will cut all of the slices for you. Due to this additional precision and automation, the VF-300 can be used for cutting fixed tissues for immunohistochemistry as well as cutting live tissues.
A: Yes, you can. You can use an icy buffer for slicing tissues, or place ice or ice packs around the buffer tray to keep the entire tray cold.
A: At this time, the buffer tank is not removable. But we are actively designing a new model with removable buffer tanks!
A: We highly recommend using the Instant Krazy Glue that is commonly used in labs. The glue comes in a red/green vial with a control tip applicator. It is best to avoid gel-like glues because the consistency of those products could damage the tissue during the glueing process.