Lung slice sectioned with Compresstome
300um slice 3 week old mouse brain
Dr. Yan Gu and Dr. Shaoyu Ge, Department of Neurobiology, SUNY at Stony Brook
We received a demo VF-300 microtome unit from Precisionary Instruments Inc and used it for 2 months. Although we have experience using a variety of microtomes from different vendors, we were excited by the VF-300’s ability to provide superior slices when combined with the recommended cutting buffer. We obtained surprisingly good electrophysiology results from even slices of relatively old (6 week) animals. The slices were very clean on the surface, and the majority of the cells were alive including those very close to the surface. As they mentioned, the model’s design is also suited to cutting small or very soft tissues. We also used it for preparing organotypic tissue cultures, and it worked well. Shown below are 300 micron hippocampal slices from a 6-week old mouse.