We obtained a VF-300 Compresstome™ about 5 months ago to use alongside a microtome that we had exclusively used for at least 5 years prior. The VF-300 is more consistent in generating uniform slices and leaves the structure of the tissue/cells more intact than the method we had been using. We have been able to extend the amount of time over which we can cut lung slices, and allows preservation of the architecture of the slice and greater viability of the tissue.”
With the Compresstome™ I was able to prepare high quality slices with excellent preservation near the slice surface. The main advantages of this machine are the rapid speed of slicing and the manual stabilization afforded by the agarose embedding. I am able to prepare uniform slices in both the coronal and horizontal planes, and I routinely complete transcardial perfusion, brain removal, and slicing all within less than 10 minutes, which is half the time needed with the Vibratome model. By limiting the time required for slicing the brain tissue is able to recover more easily without significant anoxia, and my slices stay viable for up to 8 hours. I have successfully been able to do targeted whole-cell recordings from fluorescently labeled neurons in slices prepared from >1 year old mice, which is not generally possible with conventional slicers and methods.”
We found the Compresstome produces a less apparent damage to the cells of the airways and arterioles as well as to the delicate alveolar parenchyma. Using the Compresstome we are able to obtain more reproducible and successful experiments.”
For the past several months, I have been using the Precisionary Instruments IncVF-200 to cut 300 um prefrontal cortical slices for electrophysiological recordings. As a result, I have been consistently cutting exceptional quality brain slices and have succeeded in obtaining stable whole-cell patch recordings from numerous neurons. Because I am using transgenic mice that have fluorescent reporters, it is of great importance to achieve maximal cell viability and minimal cell death. When I use the VF-200, I have significantlygreater number of surface neurons than those slices cut from a regular Vibratome™. In addition the slice surface is much more even, which allows for better imaging. A small adjustment I have made to this system is melting dental wax between the blade and the holder, which holds the blade in place sufficiently, instead of the conventional method using glue. This alleviates many of the problems associated with removing the glue off the blade holder. I am thoroughly impressed with the VF-200 and would not hesitate recommending this quality product to fellow electrophysiologists.
“We received a demo VF-300 microtome unit from Precisionary Instruments Inc and used it for 2 months. Although we have experience using a variety of microtomes from different vendors, we were excited by the VF-300’s ability to provide superior slices when combined with the recommended cutting buffer. We obtained surprisingly good electrophysiology results from even slices of relatively old (6 week) animals. The slices were very clean on the surface, and the majority of the cells were alive including those very close to the surface. As they mentioned, the model’s design is also suited to cutting small or very soft tissues. We also used it for preparing organotypic tissue cultures, and it worked well. Shown below are 300 micron hippocampal slices from a 6-week old mouse.”
Image of a lung slice sectioned with the Compresstome® tissue slicer.
Live brain slice from a 18-month-old mouse showing neurons from the hippocampal CA1 region prepared with the Compresstome® for patch-clamp electrophysiology.
brain slice from a 3-week-old mouse
Live brain slice sectioned with the Compresstome showing the hippocampal dentate gyrus region.