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Speeds & Settings Best Practices

Here to help you with getting the best slices!

You may wonder what the “best” cutting parameters are for the Compresstome®. Below are suggestions for where to get started!

 

Tissue or Specimen

Cutting Speed

Oscillation setting

Lung: Precision-cut lung slices

3-4

4-6

Any fixed tissues

1.5-2

5-7

Brain: live Electrophysiology imaging

3-4

5-7

Organotypic slices: Tumor research Brain culture slices

3-4

5-7

Retina (fixed and acute slices) * Infuse with agarose or gelatin to replace vitreous humor

1.5-2

5-7

Kidney (acute slices)

3-4

5-6

Liver (acute slices)

3-4

5-6

Cardiac (fixed and acute slices) * Infuse with agarose or gelatin to fill up atria/ventricles

1.5-2

5-6

Gut (fixed and acute slices)

4-5

4-5

Plants: Leaves

3-4

4-5

Plants: Stems

3-4

4-5

Plants: Seeds

6-8

2-3

Skin (Epidermis)

2-3

5-6

Muscle (fixed and acute slices)

2-3

4-6

Adipose (fixed and acute slices)

4-5

5-6

Lymph node (fixed and acute slices) * Use 3% agarose for more fibrous tissues

1.5-2

5-6

Spleen (fixed and acute slices)

3-4

6-7

* Please note that these cutting parameters are suggestions or starting points for your experiments. With the Compresstome®, experiment with different parameters to optimize your slices!