References & Protocols

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Mice

The mouse is the most commonly used animal model for scientific research. The Compresstome® has an extensive history of being successfully used for cutting live (fresh) and fixed mouse tissues from a wide variety of organ systems.

In particular, the Compresstome® helps to section acute brain slices that are healthier, with a greater number of live neurons for electrophysiology, optogenetics, and calcium-imaging experiments. Brain slices cut with the Compresstome® are viable for up to twice as long as those made by other tissue slicers.

For fixed tissue experiments such as immunohistochemistry (IHC) or in-situ hybridization (ISH), the Compresstome® can section slices as thin as 4µm. Fresh slices may be cut down to as thin as 20µm to 40µm in thickness for organotypic culture or tumor slice studies.

Click below for the full list of Compresstome® references using mice.

Rats

Rats have been used in research for more than 200 years to help understand human physiology and advance medicine. The Compresstome® is a durable and affordable vibrating microtome to cut healthy live slices for electrophysiology and imaging studies. The Compresstome® has also been used to make precision-cut tissues slices of lung, heart, liver, spleen, kidney, and gastrointestinal tissues.

For fixed tissue studies, the Compresstome® helps make slices as thin as 4µm for immunohistochemistry (IHC) and in-situ hybridization (ISH).

Click below for the full list of Compresstome® references using rats.

Human

The Compresstome® tissue slicer can be used to cut a wide range of organ systems for studies of the human body, including the brain, lung, heart, kidney, liver, spleen, and gastrointestinal tissues. To section large or entire specimens of human brain, the Compresstome® VF-800-0Z and VF-900-0Z are the vibrating microtome models designed for larger tissue samples. Rather than using the freeze-thaw technique common for cryostats, the Compresstome® allows fixed tissues to be cut directly without freezing.

Click below for the full list of Compresstome® references using human tissues.

Non-Human Primates

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Fundamental research on HIV, the immune system, and neurological diseases have been done in non-human primates. The Compresstome® has been used to cut non-human primate lymphoid and genital tissues for immunohistochemistry (IHC) and in-situ hybridization (ISH) studies. For non-human primate brains, the Compresstome® has been crucial in helping cut thin (down to 4 µm) slices of fixed tissue, as well as healthy live slices for electrophysiology experiments. To help accommodate tissue samples of larger sizes from non-human primates, we have created the Compresstome® VF-800-0Z and VF-900-0Z models, which can section entire primate brains as well as other organs.

Click below for the full list of Compresstome® references using non-human primates.

Fish

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The Compresstome® can be used to cut tissue slices from a wide variety of fish species, ranging from zebrafish to Atlantic salmon. Live slices made from fish are used for electrophysiology experiments to study channel currents, excitatory and inhibitory circuits, and electrical effects of temporal and spatial summation. Fish are increasingly becoming widely used as small animal models for the study of human diseases such as osteoporosis, anemia, and melanoma.

Click below for the full list of Compresstome® references using zebrafish and other species.

Chick (gallus gallus)

The chick (gallus gallus) has emerged as an excellent animal model for studies in phylogenetics and embryology, which have applications in medicine and agriculture. The chick brain is used as a model for studies of the inferior colliculus, optic tectum, and neural connections of the visual pathways. The Compresstome® tissue slicer has been successfully used to cut live (fresh) chick brain slices for electrophysiological experiments in optic tectum. The Compresstome® has also been utilized to cut fixed chick tissue for studies of neuronal morphology and projection patterns of the inferior colliculus.

Click below for the full list of Compresstome® references using chick (gallus gallus) as an animal model.

Frog

The frog has a long history of being an animal model for the study of neurodevelopment. It was used to elucidate pathways of early events such as neural plate formation and the neurophysiology of vision. The frog was central to the development of the chemoaffinity hypothesis by Roger Sperry for understanding neural wiring during development. More recently, frogs are used for studies in cloning and stem cell research. The Compresstome® can be used to section live(fresh) and fixed frog tissue.

Click below for the full list of Compresstome® references using frogs.

Guinea Pig

The Compresstome® tissue slicer has been extensively used to make healthy precision-cut lung slices, including those for the study of bronchoconstriction and airway remodeling in guinea pigs. For respiratory or pulmonary research, the guinea pig has served as a versatile animal model for studies of allergic reaction, asthma, diphtheria, and tuberculosis. Guinea pigs are commonly used to study infectious disease because of their similar immune systems to humans.

Click below for the full list of Compresstome® references using guinea pigs.

Pigs

Although not as common as mouse models, pigs have been used to develop bioprosthetic heart valves, insulin, and new surgical techniques. In experiments using the Compresstome®, pigs have been used as animal models for the study of cystic fibrosis.

Click below for the full list of Compresstome® references using pigs.

Plants

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Plants have been important as experimental models for the study of genetics and agriculture. The Compresstome® tissue slicer is able to cut slices from plant leaves, stems, roots, and seeds.

Click below for the full list of Compresstome® references using plants, as well as a basic experimental protocol for preparing plant research slices.