Mice

The mouse is the most commonly used animal model for basic sciences research, comprising over 74.7% of procedures in animal research. The Compresstome® has an extensive history of being successfully used for cutting live (fresh) and fixed mouse tissues from a wide variety of organ systems.

In particular, the Compresstome® helps to section acute brain slices that are healthier, with a greater number of live neurons for electrophysiology, optogenetics, and calcium-imaging experiments. Brain slices cut with the Compresstome® have been shown to be viable for twice as long as those made by competitor tissue slicers.

For fixed tissue experiments, such as immunohistochemistry (IHC) or in-situ hybridization (ISH), the Compresstome® can section tissues from 4µm to 2000µm. Fresh slices may be cut down to 20µm to 40µm in thickness, for organotypic culture or tumor slice studies.

Click below for the full list of Compresstome® references using mice, as well as a basic experimental protocol for preparing mouse brain slices.

Rats

Rats have been used in research for more than 200 years to help understand human physiology and medicine. For live (fresh) rat brain slices, the Compresstome® is an excellent, durable, and affordable vibratome to cut healthy slices for electrophysiology and imaging studies. Our company’s tissue slicer has also been used to make precision-cut tissues slices of lung, heart, liver, spleen, kidney, and gastrointestinal tissues.

For fixed tissue studies, the Compresstome® helps make thin slices ranging to as thin as 4µm for immunohistochemistry (IHC) and in-situ hybridization (ISH).

Click below for the full list of Compresstome® references using rat, as well as a basic experimental protocol for preparing rat brain slices

Human

The Compresstome® vibratome can be used to cut a wide range of organ systems for studies of the human body, from the human brain to lung, heart, kidney, liver, spleen, and gastrointestinal tissues. Human respiratory or pulmonary research using the Compresstome® have focused on making precision-cut lung slices. To section large or entire specimens of human brain, the Compresstome® VF-800-0Z and VF-900-0Z are the vibratome models that allow for cutting whole brain sections. Rather than using the freeze-thaw technique common for cryostats, the Compresstome® allows fixed tissues to be cut directly without freezing.

Click below for the full list of Compresstome® references using human tissue, as well as a basic experimental protocol for preparing human precision-cut lung slices.

Non-Human Primates

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Fundamental research on HIV, immunity, and neurological diseases have been done in non-human primates. The Compresstome® has been used to cut non-human primate lymphoid and genital tissues for immunohistochemistry (IHC) and in-situ hybridization (ISH) studies. For non-human primate brain, the Compresstome® has been crucial in helping cut thin (down to 4 µm) slices of fixed tissue, as well as healthy live slices for electrophysiology experiments. To help accommodate tissue samples of larger sizes from non-human primates, we have created the Compresstome® vibratome VF-800-0Z and VF-900-0Z models, which can section entire primate brains and other organs.

Click below for the full list of Compresstome® references using non-human primates, as well as a basic experimental protocol for preparing non-human primate slices.

Fish

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The Compresstome® vibratome can be used to cut tissue slices from a wide variety of fish animal models, from zebrafish to Atlantic salmon. Live slices made from fish animal models are used for electrophysiology experiments to study channel currents, excitatory and inhibitory circuits, and electrical effects of temporal and spatial summation. Fish are increasing becoming widely used as small animal models for human diseases, such as osteoporosis, anemia, and melanoma.

Click below for the full list of Compresstome® references using zebrafish and other species, as well as a basic experimental protocol for preparing fish tissue slices.

Chick (gallus gallus)

The chick (gallus gallus) has emerged as an excellent animal model for studies in phylogenetics and embryology, which have applications in medicine and agriculture. For neuroscience, the chick brain is used as a model for studies of the inferior colliculus, optic tectum, and neural connections of the visual pathways. The Compresstome® vibratome has been successfully used to cut live (fresh) chick brain slices for electrophysiological experiments in optic tectum. Our company’s tissue slicer has also been utilized to cut fixed chick tissue for studies of neuronal morphology and projection patterns of the inferior colliculus.

Click below for the full list of Compresstome® references using chick (gallus gallus) as an animal model, as well as a basic experimental protocol for preparing chick brains.

Frog

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Regeneration in the visual system of adult mammals by Yves Sauve and Frederic Gaillard

The frog has a long history as an animal model for the study of neurodevelopment. It was used to elucidate pathways of early events such as neural plate formation and the neurophysiology of vision. The frog was central to the development of the chemoaffinity hypothesis by Roger Sperry for understanding neural wiring during development. More recently, frogs are used for studies in cloning and stem cell research. The Compresstome® can be used to section frog tissue. Live frog tissue slices have been used to study the electrophysiology properties of isthmic neurons.

Click below for the full list of Compresstome® references using frog, as well as a basic experimental protocol for preparing frog neural slices.

Guinea Pig

The Compresstome® vibratome has been extensively used to make healthy precision-cut lung slices, including those for the study of bronchoconstriction and airway remodeling in guinea pigs. For respiratory or pulmonary research, the guinea pig has served as a versatile animal model for studies of allergic reaction, asthma, diphtheria, and tuberculosis. Because of their similar immune systems to humans, guinea pigs are commonly used to study infectious disease.

Click below for the full list of Compresstome® references using guinea pig, as well as a basic experimental protocol for preparing guinea pig lung slices.

Pigs

Although not as common as mouse models, pigs have been used to develop bioprosthetic heart valves, insulin, and and new surgical techniques. In experiments using the Compresstome®, pigs have been used as animal models for the study of cystic fibrosis. The Compresstome® vibratome is excellent for making precision-cut tissue slices from pig animal models.

Click below for the full list of Compresstome® references using pigs, as well as a basic experimental protocol for preparing pig precision-cut lung slices.

Plants

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Plants have been important as experimental models for the study of genetics and agriculture. The Compresstome® vibratome is able to cut slices from plant leaves, stems, roots, and seeds.

Click below for the full list of Compresstome® references using plants, as well as a basic experimental protocol for preparing plant research slices.