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Electrophysiology

Problems with other market slicers

  • Unable to stabilize tissue specimen
  • Tissue shearing during cutting
  • Shearing damages surface layer neurons
  • Lack of healthy cells for experiments

Principles of the Compresstome®

  • Stabilizes brain tissue using agarose embedding
  • Prevents thick-and-thin tissue slices
  • Results in smoothly cut, consistent slices
  • Healthier neurons for patch-clamp recording!

Electrophysiology requires healthy acute slices of tissue. The quality of your experiments will depend on the quality of the tissue slices. The Compresstome® helps create superior acute brain slices with a higher number of viable neurons for patch-clamp experiments. The Compresstome® vibrating microtome is also used to produce live cardiac and kidney slices for electrophysiology.

The Compresstome semi-automated VF-200-0Z and fully automated VF-300-0Z produce the highest quality brain slices for these researches.

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Advantages over the cryostat

Simpler Protocol: With the Compresstome® vibrating microtome, you no longer need sucrose incubation, OCT embedding, or liquid nitrogen freezing. Our simpler cutting procedures saves you time and money.

Better histology images: Tissue slices made with the Compresstome® have no ice crystal formation, which helps to preserve cellular structure for histology.

Compact size:  The footprint of the Compresstome® slice. is very small, allowing it to be easily moved in a lab without taking up space.

Affordable prices: The total cost for a Compresstome® is significantly lower than that of a cryostat. The savings on material cost and your time is even bigger.

Advantages over a traditional vibrating microtome

Easier to use:The operation of a Compresstome® is simpler than that of market vibrating slicers. We also provide full training and support to new users.

Thinner slices:With traditional vibrating slicers, tissue starts shredding around 50µm. With the Compresstome®, you get smooth and consistently-sliced sections down to 4µm.

No chatter marks: In the Compresstome®, chatter marks or vibration artifacts are eliminated from the slice surface by our patented Auto Zero-Z technology.

Faster cutting: The Compresstome® can cut up to 5X faster than other vibrating slicers.

Precision Cut Lung Slices

Allows researchers to observe and assess organ functions under cell culture conditions to study the metabolism of almost any organ system.

Compresstome® is ideal for all precision-cut organ system:

  • Lung
  • Liver
  • Intestines
  • Spleen
  • Kidney
  • Lymph nodes
  • Thymus
  • Skeletal muscle
  • Adipose tissue
  • Heart

Precision-cut tissue slices require healthy and viable sections to be made for metabolism studies. Slicing is the first and most important step of this procedure. The ability to cut consistent tissue slices to minimize cellular damage is crucial for subsequent metabolism experiments.

With our patented Auto Zero-Z technology, the Compresstome® vibrating microtome can create healthier precision-cut tissue slices with an increased number of healthy or viable cells of studies of metabolism, pharmacology, and toxicology.

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In Situ Hybridization

Proper tissue fixture and preparation is crucial for getting the best in-situ hybridization (ISH) signals. Our Compresstome® allows you to cut thin free-floating sections down to 4 µm-thick, but without having to freeze any tissue. Slices over 10 µm-thick present problems for ISH staining, and the Compresstome® is the only vibrating microtome on the market that can section free-floating slices for ISH! Avoid freeze artifacts that can interfere with sensitive ISH staining processes by using a Compresstome®.

The VF-310-0Z with the Auto Zero-Z® feature is the recommended Compresstome®for in-situ applications.

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Fresh cervical tissue cut with a Compresstome® VF-300-0Z and processed for in-situ tetramer staining combined with immunohistochemistry.

Organotypic Cultures

The key to performing the best tissue culture experiments is to use healthy tissue slices. Traditional slicing with tissue choppers cause tearing and stretching of samples. Our Compresstome® is able to hold your tissue sample firmly in place while sectioning, providing maximum support. This produces perfect slices for slice culture experiments.

With our patented compression technology, we firmly hold the tissue in place when the sectioning is performed, providing tissue with the maximum support.  This is minimally invasive to the sub-cellular structures and provides the best slices for organotypic tissue culture.

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