References & Protocols

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Brain (Fixed)

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Mouse hippocampus slice (40µm) stained for BrdU (red), GFP (green), and DAPI(blue).

H&E staining of mouse cerebellum sectioned using Compresstome® .

Studies of animal brains have influenced our knowledge of human development, and helped us develop new and fascinating treatments for neurodegenerative diseases.

The Compresstome® has a proven history with successfully sectioning fixed brain tissue, providing unique benefits over other market vibrating microtome. Trusted by the Allen Institute for Brain Science, the Compresstome® outperforms the competition by producing tissue slices without chattermarks. Slices made with the Compresstome® also produce consistent slice thicknesses at a faster cutting speed, saving you time for experiments.

● For fixed tissue experiments, such as immunohistochemistry (IHC) or in-situ hybridization (ISH), the Compresstome® can section tissue from 4µm.

● For live tissue experiments, such a electrophysiology or imaging, our vibrating microtome can section tissues from 20µm to 2000µm.

Click below for the full list of Compresstome® references using fixed brain tissue, as well as a basic experimental protocol for preparing fixed brain sections.

Brain (Live/Fresh)

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Live cortex slice for patch-clamp electrophysiology from an 18-month-old mouse.

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The Compresstome® has been widely used by some of the top researchers around the world to study brain tissue, such as the Allen Institute for Brain Science. Slicing soft tissue such as fresh brain present unique challenges that many vibrating microtomes struggle to overcome, but the Compresstome® has been used to section brains from neonatal mice to mice as old as 24 months! Thus, our vibrating microtome is useful for studies of neurodevelopment in young animal models, as well as for neurodegenerative diseases in mature animal models, which are especially difficult to cut.

When slicing fresh brain tissue for the purposes of patch-clamp electrophysiology, it is important to preserve as many healthy surface neurons as possible in order to provide the best results for your research.

The Compresstome’s® patented technology helps stabilize the tissue with a gentle compression effect during cutting which enables it to section fresh brain tissue with smoother tissue surfaces and healthier neurons for recordings. Brain slices cut with the Compresstome® have been shown to have increased survival time, often over twice as long as slice cut with other market tissue slicer.

Click below for the full list of Compresstome® references using fresh brain tissue, as well as a basic experimental protocol for preparing fresh brain sections.

Heart

Human heart and micrograph with closeup view of cardiac muscle structure, 3D illustration and photo under microscope
Heart hypertrophy. Photomicrograph showing hypertrophic myocardium with thick muscle fibers and enlarged and dark nuclei

Often lumped together as just “cardiovascular disease”, the heart can suffer from a number of diseases of various causes such as atherosclerosis, coronary artery disease, arrhythmias, valve disorders, and any others. Recent estimates from the American Heart Association estimates that more than 92 million American adults are living with some form of heart disease.

For fixed cardiac tissue experiments, such as immunohistochemistry (IHC) or in-situ hybridization (ISH), the Compresstome® can section tissues from 4µm to 2000µm. Fresh slices may be cut down to 20µm to 40µm in thickness. The Compresstome® is able to section heart tissue over 5x faster than other leading market tissue slicer, helping to keep fresh heart tissue alive and prevent ischemia for electrophysiology experiments

Video tutorial of how to cut live cardiac slices with the Compresstome®

Click below for the full list of Compresstome® references using heart tissue, as well as a basic experimental protocol for preparing heart slices.

Tumor

Cancer is one of the most common diseases in the world. The ongoing search for a cure constitutes one of the largest collective global endeavors in human history.

With such important research being conducted, it is paramount that scientists have access to the best equipment available. Most market vibrating microtomes have difficulty providing quality slices of fresh tissue below 100µm, but the Compresstome® has been proven to section fresh tissue as low as 20µm to 40µm in thickness while providing smooth surfaces free of chattermarks.Tumor slices made with the Compresstome® can be used for imaging, organotypic cultures, and many other applications.

Click below for the full list of Compresstome® references sectioning tumors, as well as a basic experimental protocol for preparing tumor slices for sectioning

Liver

Realistic illustration of comparsion of healthy and sick (cirrhosis) human livers

The liver is far more than just the largest organ in the human body. The liver performs many vital functions such as detoxifying the body, manufacturing and maintaining cholesterol, mediating pathways like glycolysis and gluconeogenesis, and synthesizing vital proteins. Researchers study a multitude of diseases affecting the liver, including viral hepatitis, alcohol induced cirrhosis, or a plethora of metabolic diseases.

The Compresstome®, can section fixed liver tissue to as thin as 4µm. Fixed liver slices can then be processed for immunohistochemistry (IHC), in-situ hybridization (ISH), or H&E staining. For studies of metabolism, fresh liver can be cut with the Compresstome® to give you healthy precision-cut liver slices.

Click below for the full list of Compresstome® references using liver tissue, as well as a basic experimental protocol for preparing liver tissue for sectioning.

Click below for the full list of Compresstome® references using liver tissue, as well as a basic experimental protocol for preparing liver tissue for sectioning.

Lung

3D illustration of Lungs - Part of Human Organic.

Respiratory or pulmonary research focuses on developing new therapies for people with lung diseases such as asthma, chronic obstructive pulmonary disease (COPD), pulmonary fibrosis, cystic fibrosis, and pulmonary arterial hypertension. Researchers can use precision-cut lung slices (PCLS) for studying the biochemical pathways of respiratory disorders, and for testing the effects of various drugs on bronchodilation or bronchoconstriction.

The Compresstome® is the most widely used tissue slicer in the United States for making precision-cut lung slices, providing healthier tissue for studies of respiratory physiology. For studies of lung anatomy and microstructures, the Compresstome® tissue slicer can section lung tissue as thin as 4µm. Fixed lung slices can then be processed for immunohistochemistry (IHC), in-situ hybridization (ISH), or H&E staining.

For video tutorials and demonstrations of the Compresstome® for PCLS, please visit:
https://www.jove.com/video/55465/precision-cut-mouse-lung-slices-to-visualize-live-pulmonary-dendritic

Click below for the full list of Compresstome® references using lung tissue, as well as a basic experimental protocol for preparing precision-cut lung slices.

Lymph Nodes & Spleen

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Anthracotic lymph node. Accumulation of carbon is most commonly found in intrapulmonary lymph nodes, due to coal dust, smoke or pollution.  Hematoxylin & eosin stain
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Studies of the lymphatic system are crucial to better understand the immune system. Lymph nodes, rich in B and T white blood cells, function as a filtration system for waste products, bacteria, foreign objects, damaged or abnormal cells, and helping to fight infections. Serving as a key part in the diagnosis and treatment of many types of cancers, researchers study the lymphatic system to aid in the ongoing fight against cancer.

Considered an invaluable tool for many researchers, the Compresstome® can section fixed lymph nodes as thin as 4µm. Fixed lymph node or spleen slices can then be processed for immunohistochemistry (IHC), in-situ hybridization (ISH), or H&E staining. Click below for the full list of Compresstome® references using lymph node tissue, as well as a basic experimental protocol for preparing lymph node tissue.

Click below for the full list of Compresstome® references using lymph node tissue, as well as a basic experimental protocol for preparing lymph node tissue.

Muscle

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Muscle tissue research focuses largely around diseases affecting skeletal and smooth muscle tissue. However, scientists studying muscle have recently gained insights that could create new treatments for many muscular disorders.

Muscle tissue research is conducted on a variety of animals including humans, mice, rats, and many others. The Compresstome® has a proven history of handling difficult and fibrous tissue that other vibrating microtomes struggle with. Our patented compression technology helps to stabilize the tissue during the cutting process which allows the Compresstome® to produce better slices at faster speeds when compared to the competition. The Compresstome® can section fixed tissue as thin as 4µm, and fresh tissue as thin as 20µm to 40µm.

Click below for the full list of Compresstome® references using muscle tissue, as well as a basic experimental protocol for preparing muscle tissue.

Reproductive Tissue

Corpus albicans. Human ovary. The corpus albicans is the regressed form of the corpus luteum. After the corpus luteum degenerates, it is replaced by white scar of fibrous tissue made by type I collagen.
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Reproductive tissue represents one of the most valuable resources for providing pluripotent stem cells. Researchers rely heavily on sources of reproductive tissue such as ovarian, testicular, and embryonic stem cells. Studies into reproductive tissues and stem cells have greatly advanced our collective knowledge about the developmental process.

The Compresstome® is able to section fixed tissue as thin as 4µm. Sectioning up to 5x faster than the competition, the Compresstome® will enhance the speed of your workflow while providing you with smooth and healthy slices free from chattermarks.

Click below for the full list of Compresstome® references using reproductive tissue, as well as a basic experimental protocol for preparing reproductive tissue.

Spinal Cord

Trusted by some of the top researchers in the field such as the Allen Institute for Brain Science, the Compresstome® provides many benefits over the competition for both fresh and fixed tissue.

The Compresstome® has patented technology that enables it to section fresh tissue for electrophysiology with much greater ease. The Compresstome® provides smoother, healthier slices by reducing the damage done to the surface neurons which greatly improves your patch clamping results.

Click below for the full list of Compresstome® references using spinal cord tissue, as well as a basic experimental protocol for preparing spinal cord tissue.