Brain (Fixed)

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Source: Precisionary Instruments.
Legend: Mouse hippocampus slice (40µm) stained for BrdU (red), GFP (green), and DAPI(blue).

Source: Precisionary Instruments.
Legend: H&E staining of mouse cerebellum sectioned using Compresstome® vibratome.

Studies of animal brains have influenced our knowledge of human development, and helped us develop new and fascinating treatments for neurodegenerative diseases.

The Compresstome® has a proven history with successfully sectioning fixed brain tissue, providing unique benefits over other market vibratomes. Trusted by the Allen Institute for brain science, the Compresstome® outperforms the competition by producing tissue slices without chattermarks. Slices made with the Compresstome® also produce consistent slice thicknesses at a faster cutting speed, saving you time for your experiments.

● For fixed tissue experiments, such as immunohistochemistry (IHC) or in-situ hybridization (ISH), the Compresstome® can section tissues from 4µm to 2000µm.

● For live tissue experiments, such a electrophysiology or imaging, our vibratome can section tissues from 20µm to 2000µm.

Click below for the full list of Compresstome® references using fixed brain tissue, as well as a basic experimental protocol for preparing fixed brain sections.

Brain (Live/Fresh)

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Source: Jonathan Ting, Allen Institute for Brain Science.
Legend: Live cortex slice for patch-clamp electrophysiology from an 18-month-old mouse.

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Source: Precisionary Instruments.

**Also include Allen Institute patch clamp video, same as electrophysiology

The Compresstome® has been widely used by some of the top researchers around the world to study brain tissue, such as the Allen Institute for brain science. Slicing soft tissue such as fresh brain tissue can present some unique challenges that many vibratomes struggle to overcome, but the Compresstome® has been used to section tissue ranging from neonatal mice to mice as old as 24 months! Thus, our vibratome is useful for studies of neurodevelopment in young animal models, as well as neurodegenerative diseases in mature animal models, which are especially difficult to cut.

When slicing fresh brain tissue for the purposes of patch-clamp electrophysiology, it is important to preserve as many healthy surface neurons as possible in order to provide the best results for your research.

The Compresstome’s® patented technology helps stabilize the tissue with a gentle compression effect during cutting which enables it to section fresh brain tissue with smoother tissue surfaces and healthier neurons for recordings. Brain slices cut with the Compresstome® have been shown increased survival time, often over twice as long as slice cut with other market vibratomes.

Click below for the full list of Compresstome® references using fresh brain tissue, as well as a basic experimental protocol for preparing fresh brain sections.

Heart

Heart hypertrophy. Photomicrograph showing hypertrophic myocardium with thick muscle fibers and enlarged and dark nuclei

Often lumped together as just “cardiovascular disease”, the heart can suffer from a number of diseases of various causes such as atherosclerosis, coronary artery disease, arrhythmias, valve disorders, and any others. Recent estimates from the American Heart Association estimates that more than 92 million American adults are living with some form of heart disease.

For fixed cardiac tissue experiments, such as immunohistochemistry (IHC) or in-situ hybridization (ISH), the Compresstome® can section tissues from 4µm to 2000µm. Fresh slices may be cut down to 20µm to 40µm in thickness. The Compresstome® is able to section heart tissue over 5x faster than other leadin market vibratomes, helping to keep fresh heart tissue alive and prevent ischemia for electrophysiology experiments

For a video tutorial of how to cut live cardiac slices with the Compresstome®, please visit: https://www.youtube.com/watch?v=Q0PT37BPles

Click below for the full list of Compresstome® references using heart tissue, as well as a basic experimental protocol for preparing heart slices.

Tumor

Cancer is one of the most common diseases in the world. Considered by many as one of the diseases which links us all together as it is not limited by age, gender, class, or ethnicity, the ongoing search for a cure constitutes one of the largest collective global endeavors in human history.

With such important research being conducted, its paramount that scientists have access to the best equipment available. Most market vibratomes have difficulty providing quality slices of fresh tissue below 100µm, but the Compresstome® has been proven to section fresh tissue as low as 20µm to 40µm in thickness while providing smooth surfaces free of chattermarks to greatly improve your imaging quality. Tumor slices made with the Compresstome® can be used for imaging, organotypic cultures, and many other applications.

Click below for the full list of Compresstome® references sectioning tumors, as well as a basic experimental protocol for preparing tumor slices for sectioning

Liver

Realistic illustration of comparsion of healthy and sick (cirrhosis) human livers

The liver is far more than just the largest organ in the human body. The liver functions like an airport hub, that almost all biochemical pathways in animals pass through at one point or another. Performing many vital functions such as detoxifying the body, manufacturing and maintaining cholesterol, triglycerides, glycolysis and gluconeogenesis, and synthesis of many vital proteins, among numerous other functions. One of the most vital and complex organs in the body, diseases which affect hepatic tissue such as viral hepatitis, alcohol induced cirrhosis, or a plethora of metabolic diseases are studied by researchers around the globe.

For studies of Liver anatomy and microstructures, the Compresstome® vibratome can section liver tissue with slice thickness ranging from 4µm to 2000µm. Fixed liver slices can then be processed for immunohistochemistry (IHC), in-situ hybridization (ISH), or H&E staining. For studies of metabolism, live liver slices can be cut with the Compresstome® to give you healthy precision-cut liver slices.

Click below for the full list of Compresstome® references using liver tissue, as well as a basic experimental protocol for preparing liver tissue for sectioning.

Lung

3D illustration of Lungs - Part of Human Organic.

Respiratory or pulmonary research focuses on developing new therapies for people with lung diseases, such as asthma, chronic obstructive pulmonary disease (COPD), pulmonary fibrosis, cystic fibrosis, and pulmonary arterial hypertension. Basic sciences research typically use precision-cut lung slices (PCLS) for studying the biochemical pathways of respiratory disorders, and for testing the effects of drugs on bronchodilation or bronchoconstriction. PCLS from a variety of animal models (mice, rats, pig, sheep, human) serve as a phenotypically-accurate functional assay system for pulmonary research.

The Compresstome® vibratome is the most widely used tissue slicer in the United States for making precision-cut lung slices for testing xenobiotics, chemicals, and medications.

The Compresstome® VF-300-0Z is the recommended model for slicing PCLS, which provides lung slices with healthier tissue that can be kept in culture for up to multiple days. For studies of lung anatomy and microstructures, the Compresstome® vibratome can section lung tissue with slice thickness ranging from 4µm to 2000µm. Fixed lung slices can then be processed for immunohistochemistry (IHC), in-situ hybridization (ISH), or H&E staining.

For video tutorials and demonstrations of the Compresstome® for PCLS, please visit:

https://www.jove.com/video/55465/precision-cut-mouse-lung-slices-to-visualize-live-pulmonary-dendritic

Click below for the full list of Compresstome® references using lung tissue, as well as a basic experimental protocol for preparing precision-cut lung slices.

Lymph Nodes & Spleen

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Anthracotic lymph node. Accumulation of carbon is most commonly found in intrapulmonary lymph nodes, due to coal dust, smoke or pollution. Hematoxylin & eosin stain
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Studies of the lymphatic system are crucial to better understand the immune system. Lymph nodes, rich in B and T white blood cells, function as a filtration system for waste products, bacteria, foreign objects, damaged or abnormal cells such as cancer cells, as well as helping to fight infections.

Clinically speaking, lymph nodes provide medical doctors and researchers with valuable information for not just lymphomas but for all types of secondary cancers all over the body.Serving as a key part in the diagnosis and treatment of many types of cancers, researchers have and will continue to utilize the study of the lymphatic system in the ongoing fight against cancer.

Considered an invaluable tool for many researchers of the lymphatic system, the Compresstome® vibratome can section fixed lymph node tissue with slice thickness ranging from 4µm to 2000µm. Fixed lymph node or spleen slices can then be processed for immunohistochemistry (IHC), in-situ hybridization (ISH), or H&E staining.

Click below for the full list of Compresstome® references using lymph node tissue, as well as a basic experimental protocol for preparing lymph node tissue.

Muscle

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Muscle tissue research focuses largely around various muscular diseases affecting skeletal and/or smooth muscle tissue. However, scientists studying muscle have recently gained new insights that could create new treatments for muscular disorders. In 2015, a team of researchers was able to generate new human skeletal muscle tissue from induced pluripotent stem cells made from human skin cells.

Muscle tissue research is conducted on a variety of animals including humans, mice, rats, and others. For smooth, healthy muscle tissue sectioning, the Compresstome® has a proven history of handling difficult and fibrous tissue that other vibratomes struggle with. Our patented compression technology helps stabilize the tissue during the cutting process allowing our tissue slicer to produce better slices and at faster speeds when compared to the competition. The Compresstome® can section fixed tissue from 4µm to 2000µm, and fresh tissue from 20µm to 2000µm.

Click below for the full list of Compresstome® references using muscle tissue, as well as a basic experimental protocol for preparing muscle tissue.

Reproductive Tissue

Corpus albicans. Human ovary. The corpus albicans is the regressed form of the corpus luteum. After the corpus luteum degenerates, it is replaced by white scar of fibrous tissue made by type I collagen.
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Reproductive tissue represents the most valuable resource for providing pluripotent stem cells that we know of. Stem cell researchers rely heavily on sources of reproductive tissue to provide them, such as ovarian, testicular, and embryonic stem cells. Studies into reproductive tissues and stem cells have been greatly advancing our collective knowledge about the developmental process due to the effect of variable conditions such as hormones.

As with all soft tissue, the Compresstome® provides a key benefit over other market vibratomes. Able to section fixed tissue as thin as 4µm, the Compresstome® does what the others cant. Sectioning over 5x faster than the competition, the Compresstome® will enhance the speed of your workflow while providing you with smooth, chattermark free tissue slices!

Click below for the full list of Compresstome® references using reproductive tissue, as well as a basic experimental protocol for preparing reproductive tissue.

Spinal Cord

Neuroscience involves more than just the study of the brain! Pain researchers study of the central nervous system can’t be completed without advancing our knowledge of spinal cord tissue. Trusted by some of the top researchers in the field such as the Allen Institute for Brain Science, the Compresstome® provides real tangible benefits over the competition whether it be for live slicing for the purposes of electrophysiology or fixed tissue slicing for immunohistochemistry.

The Compresstome’s® patented technology enables it to section fresh tissue for the purposes of electrophysiology with much greater ease. The Compresstome® provides smoother, healthier slices reducing the damage done to the surface neurons by most market vibratomes, which greatly improves your patch clamping results.

Click below for the full list of Compresstome® references using spinal cord tissue, as well as a basic experimental protocol for preparing spinal cord tissue.