Compresstome® Cutting Parameters
| Tissue or Specimen | Advance (Speed) | Oscillation | Suggested Agarose % |
|---|---|---|---|
Lung:
| 2-3 | 3-4 | 2% |
| Any fixed tissues | 1.5-2 | 3-4 | 2% |
Brain: live
| 1.5-2 | 3-4 | 1.8-2% |
| Organotypic slices: Tumor research Brain culture slices | 2-3 | 4-5 | 2% |
| Retina (fixed and acute slices) * Infuse with agarose or gelatin to replace vitreous humor | 1.5-2 | 5-6 | 2.5% |
| Kidney (acute slices) | 3-4 | 5-6 | 2.5% |
| Liver (acute slices) | 3-4 | 5-6 | 2.5% |
| Cardiac (fixed and acute slices) * Infuse with agarose or gelatin to fill up atria/ventricles | 1.5-2 | 5-6 | 2.5% |
| Gut (fixed and acute slices) | 3-4 | 3-4 | 2.5% |
| Plants: Leaves | 3-4 | 4-5 | 2% |
| Plants: Stems | 3-4 | 4-5 | 2% |
| Plants: Seeds | 6-8 | 2-3 | 2.5% |
| Skin (Epidermis) | 2-3 | 5-6 | 2.5% |
| Muscle (fixed and acute slices) | 2-3 | 4-6 | 2.5% |
| Adipose (fixed and acute slices) | 4-5 | 5-6 | 1.8% |
| Lymph node (fixed and acute slices) * Use 3% agarose for more fibrous tissues | 1.5-2 | 5-6 | 2.5% |
| Spleen (fixed and acute slices) | 3-4 | 6-7 | 2% |