Compresstome® Cutting Parameters
Tissue or Specimen | Advance (Speed) | Oscillation | Suggested Agarose % |
---|---|---|---|
Lung:
| 2-3 | 3-4 | 2% |
Any fixed tissues | 1.5-2 | 3-4 | 2% |
Brain: live
| 1.5-2 | 3-4 | 1.8-2% |
Organotypic slices: Tumor research Brain culture slices | 2-3 | 4-5 | 2% |
Retina (fixed and acute slices) * Infuse with agarose or gelatin to replace vitreous humor | 1.5-2 | 5-6 | 2.5% |
Kidney (acute slices) | 3-4 | 5-6 | 2.5% |
Liver (acute slices) | 3-4 | 5-6 | 2.5% |
Cardiac (fixed and acute slices) * Infuse with agarose or gelatin to fill up atria/ventricles | 1.5-2 | 5-6 | 2.5% |
Gut (fixed and acute slices) | 3-4 | 3-4 | 2.5% |
Plants: Leaves | 3-4 | 4-5 | 2% |
Plants: Stems | 3-4 | 4-5 | 2% |
Plants: Seeds | 6-8 | 2-3 | 2.5% |
Skin (Epidermis) | 2-3 | 5-6 | 2.5% |
Muscle (fixed and acute slices) | 2-3 | 4-6 | 2.5% |
Adipose (fixed and acute slices) | 4-5 | 5-6 | 1.8% |
Lymph node (fixed and acute slices) * Use 3% agarose for more fibrous tissues | 1.5-2 | 5-6 | 2.5% |
Spleen (fixed and acute slices) | 3-4 | 6-7 | 2% |