Compresstome® Cutting Parameters

Tissue or Specimen Advance
(Speed)		 Oscillation Suggested
Agarose %
Lung:
  • Precision-cut lung slices
 3-4 4-6 2%
Any fixed tissues 1.5-2 5-7 2%
Brain: live
  • Electrophysiology
  • imaging
 3-4 5-7 1.8-2%
Organotypic slices:
Tumor research Brain culture slices		 3-4 5-7 2%
Retina (fixed and acute slices)
* Infuse with agarose or gelatin
to replace vitreous humor		 1.5-2 5-7 2.5%
Kidney (acute slices) 3-4 5-6 2.5%
Liver (acute slices) 3-4 5-6 2.5%
Cardiac (fixed and acute slices)
* Infuse with agarose or gelatin
to fill up atria/ventricles		 1.5-2 5-6 2.5%
Gut (fixed and acute slices) 4-5 4-5 2.5%
Plants: Leaves 3-4 4-5 2%
Plants: Stems 3-4 4-5 2%
Plants: Seeds 6-8 2-3 2.5%
Skin (Epidermis) 2-3 5-6 2.5%
Muscle (fixed and acute slices) 2-3 4-6 2.5%
Adipose (fixed and acute slices) 4-5 5-6 1.8%
Lymph node (fixed and acute slices)
* Use 3% agarose for more fibrous tissues		 1.5-2 5-6 2.5%
Spleen (fixed and acute slices) 3-4 6-7 2%