Experiment: Precision-Cut Tissue Slices (PCTS)

Precision Cut Lung Slices: Basic Principles and Applications

Ken Chmiel (pronounced Shumel) is a Staff Research Associate at the University of California, Davis, with over 30 years of experience in mammalian cell culture. He first trained in the preparation of precision-cut lung slices (PCLS) at Harvard University in the lab of Dr. Xinbin Ai, in collaboration with Dr. Yan Bai. Since then, Ken […]

Written by on September 18, 2025

Cellular and Molecular Dynamics of Lung Injury and Repair

Zachary Saracino received his B.S. in Biochemistry in 2022 and his M.S. in Molecular and Cell Biology in 2023. He joined the Chioccioli Lab at Yale School of Medicine in 2024, where he currently works as a Postgraduate Associate in the Departments of Comparative Medicine and Genetics. Zach’s research centers on regenerative medicine, with a particular focus on understanding how alveolar stem cells behave during injury and repair in the lung.

Written by on August 19, 2025

Precision Cut Liver Slice from Healthy and Diseased Human Liver Tissue

Dr. Z. Gordon Jiang is a transplant hepatologist and physician-scientist at Beth Israel Deaconess Medical Center and Harvard Medical School. His research investigates lipid metabolism in liver diseases such as alcohol-associated liver disease (ALD) and metabolic dysfunction-associated steatotic liver disease (MASLD). Dr. Jiang’s lab develops human cell-based liver models that illuminate how hepatocyte differentiation and regeneration are altered in disease, with the ultimate goal of identifying new therapeutic strategies.
Over the past two years, his team has advanced the use of Precision-Cut Liver Slices (PCLS), leveraging both healthy and diseased human tissues obtained via liver transplantation. In this webinar, Dr. Jiang will present technical refinements in the PCLS platform, insights into the biology of liver slices in culture, and emerging applications in translational research.

Written by on July 8, 2025

The Use of Precision-Cut Lung Slices in Pulmonary Fibrosis

Dr. Johad Khoury is a physician-scientist specializing in internal medicine and pulmonary medicine. After earning his M.D. from the Technion School of Medicine in Israel, he completed his internship and residency at Rambam Health Care Campus, followed by a clinical fellowship in pulmonary diseases at Carmel Medical Center. He later became a senior physician at Carmel, where he established the first post-COVID clinic in northern Israel, and developed the pulmonary division at EMMS Nazareth Hospital.

Written by on June 3, 2025

Fundamentals of Plant Cryopreservation: Tracking the Permeation of Cryoprotectants into Living Shoot Tip Sections

In this webinar, Heidi Kreckel will present key findings from her Ph.D. research on tracking cryoprotectant permeation into living peppermint (Mentha x piperita) shoot tips. She will also discuss the use of a vibrating microtome to prepare plant shoot tip sections for live-tissue microscopy experiments, highlighting how the Compresstome vibratome facilitates precise sectioning for high-resolution imaging.​

Written by on March 6, 2025

Immunology and Infection: The Compresstome® for Precision-Cut Lung Slices

The Compresstome® has been widely used by researchers worldwide for making precision-cut lung slices (PCLS). The Compresstome® uses agarose embedding prior to slicing to allow for the preservation of open alveoli and better tissue compliance. The video above shows Compresstome® sectioning PCLS for immunostaining to visualize the localization of various immune cell types in the lung. This protocol can be extended […]

Written by on April 25, 2023