Tissue or Specimen

Cutting Speed

Oscillation setting

  Lung: Precision-cut lung slices

3-4

4-6

  Any fixed tissues

1.5-2

5-7

  Brain: Live Electrophysiology imaging

3-4

5-7

 Organotypic slices: Tumor research, Brain culture slices

3-4

5-7

  Retina (fixed and acute slices)

 * Infuse with agarose or gelatin to replace vitreous humor

1.5-2

5-7

  Kidney (acute slices)

3-4

5-6

  Liver (acute slices)

3-4

5-6

  Cardiac (fixed and acute slices)

 * Infuse with agarose or gelatin to fill up atria/ventricles

1.5-2

5-6

  Gut (fixed and acute slices)

4-5

4-5

  Plants: Leaves

3-4

4-5

  Plants: Stems

3-4

4-5

  Plants: Seeds

6-8

2-3

  Skin (Epidermis)

2-3

5-6

  Muscle (fixed and acute slices)

2-3

4-6

  Adipose (fixed and acute slices)

4-5

5-6

  Lymph node (fixed and acute slices)

  * Use 3% agarose for more fibrous tissues

1.5-2

5-6

  Spleen (fixed and acute slices)

3-4

6-7