Here to help you with getting the best slices
You may wonder what the “best” cutting parameters are for the Compresstome®. Below are suggestions for where to get started!
Tissue or Specimen | Cutting Speed | Oscillation setting |
Lung: Precision-cut lung slices | 3-4 | 4-6 |
Any fixed tissues | 1.5-2 | 5-7 |
Brain: live Electrophysiology imaging | 3-4 | 5-7 |
Organotypic slices: Tumor research Brain culture slices | 3-4 | 5-7 |
Retina (fixed and acute slices) * Infuse with agarose or gelatin to replace vitreous humor | 1.5-2 | 5-7 |
Kidney (acute slices) | 3-4 | 5-6 |
Liver (acute slices) | 3-4 | 5-6 |
Cardiac (fixed and acute slices) * Infuse with agarose or gelatin to fill up atria/ventricles | 1.5-2 | 5-6 |
Gut (fixed and acute slices) | 4-5 | 4-5 |
Plants: Leaves | 3-4 | 4-5 |
Plants: Stems | 3-4 | 4-5 |
Plants: Seeds | 6-8 | 2-3 |
Skin (Epidermis) | 2-3 | 5-6 |
Muscle (fixed and acute slices) | 2-3 | 4-6 |
Adipose (fixed and acute slices) | 4-5 | 5-6 |
Lymph node (fixed and acute slices) * Use 3% agarose for more fibrous tissues | 1.5-2 | 5-6 |
Spleen (fixed and acute slices) | 3-4 | 6-7 |
