Electron microscopy is a powerful imaging technique that uses beams of electrons to visualize the ultrastructure of biological samples at high resolution. It is an essential tool in cell biology, neurobiology, and materials science, providing insights into the architecture and function of biological and non-biological materials at the nanoscale. Tissue sections are required for electron microscopy because they allow the sample to be thin enough for electrons to pass through, and provide a flat surface for imaging. Without tissue sections, the sample would be too thick for electrons to penetrate, resulting in a blurry image.
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Kim Y, Jang YN, Kim JY, Kim N, Noh S, Kim H, Queenan BN, Bellmore R, Mun JY, Park H, Rah JC, Pak DTS, Lee KJ. Microtubule-associated protein 2 mediates induction of long-term potentiation in hippocampal neurons. FASEB J. 2020 May;34(5):6965-6983. doi: 10.1096/fj.201902122RR. Epub 2020 Apr 1. PMID: 32237183. Download PDF
Friedrichsen K, Ramakrishna P, Hsiang JC, Valkova K, Kerschensteiner D, Morgan JL. Reconstructing neural circuits using multiresolution correlated light and electron microscopy. Front Neural Circuits. 2022 Oct 21;16:753496. doi: 10.3389/fncir.2022.753496. PMID: 36338333; PMCID: PMC9635852. Download PDF
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