Fluorescence microscopy is a technique that uses fluorescent molecules to visualize the location and activity of specific molecules within cells and tissues. Calcium imaging, on the other hand, is a technique that uses fluorescent indicators to monitor changes in calcium ion concentration, a crucial signaling molecule in cells. Both techniques are essential tools in neuroscience and cell biology research, allowing scientists to study the activity of individual cells and networks in real-time. Tissue sections are often required for these techniques as they provide a thin and flat sample that can be easily visualized using the fluorescent labels. Without tissue sections, it would be difficult to identify and distinguish individual cells and their activity patterns, making it challenging to study neural and cellular signaling.
Tissue sections are commonly used in confocal imaging due to their advantages in sample thickness control, improved imaging quality, ease of sample preparation and handling, compatibility with confocal microscopy, and ability to support multichannel imaging.
Tissue sections are also used in calcium imaging studies to investigate the spatial and temporal dynamics of calcium signaling within tissues. Calcium imaging is a technique that uses fluorescent calcium indicators to monitor changes in intracellular calcium levels, which play a crucial role in many physiological processes, including cell signaling, muscle contraction, and neural activity.
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Dr Astero Klampatsa (PhD) is a Team Leader in Cancer Immunotherapy at the Institute of Cancer Research, London, UK and a Senior Lecturer in King’s College London, UK. She focuses on developing novel CAR T cell therapies for mesothelioma and lung cancer, as well as the immunobiology of these malignancies for identification of markers of response to immunotherapy. In this webinar, Dr. Klampatsa will discuss how the Compresstome® was used to create precision-cut tumor slices (PCTS) as an ex vivo model for immunotherapy research.
Visikol is a contract research services company focused on leveraging advanced imaging, 3D cell culture assays and digital pathology to accelerate the drug discovery and development process. In this webinar, Visikol explains the need for in vitro liver models to study livery injury. They demonstrate the standard assay format for creating precision-cut liver slices (PCLS), and explain how the Compresstome® VF-310-0Z vibrating microtome helps create uniform tissue slices that can be meaningfully compared between treatments. Visikol goes through how to use the Compresstome® step-by-step for making PCLS.
The footage above shows how neuroscientists use the Compresstome® tissue slicer to obtain brain slices for imaging studies. When using the Compresstome® tissue is embedded inside one of the specimen tubes. The specimen tube is made of two pieces: the inner plastic plunger and the outer metal tube. The inner plastic plunger is where you will mount your sample. The plunger is drawn down for your tissue sample to be embedded inside the metal tube. Please visit our website to learn more about our unique tissue slicers.
Dr. Tsilingiri is working on tumor immunotherapy and using the Compresstome vibrating microtome to examine the interaction between tumor tissues and autologous lymph node cells in slice cultures. This work is being carried out in the frame of an EU-funded Consortium, Tumour-LNoC (Tumour-Lymph node on a chip), with the ultimate goal of mimicking the metastatic process on a chip and monitor metastasizing cells in real time.
Dr. Wong shares how he built a custom-made Compresstome® for high-speed histological 3D imaging of whole organs like brains.
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Parlakgül G, Arruda AP, Pang S, Cagampan E, Min N, Güney E, Lee GY, Inouye K, Hess HF, Xu CS, Hotamışlıgil GS. Regulation of liver subcellular architecture controls metabolic homeostasis. Nature. 2022 Mar;603(7902):736-742. Epub 2022 Mar 9. PMID: 35264794; PMCID: PMC9014868. Download PDF
Wang C, Hyams B, Allen NC, Cautivo K, Monahan K, Zhou M, Dahlgren MW, Lizama CO, Matthay M, Wolters P, Molofsky AB, Peng T. Dysregulated lung stroma drives emphysema exacerbation by potentiating resident lymphocytes to suppress an epithelial stem cell reservoir. Immunity. 2023 Mar 14;56(3):576-591.e10. Epub 2023 Feb 22. PMID: 36822205. Download PDF
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