In-situ hybridization is a technique that uses nucleic acid probes to detect specific DNA or RNA sequences within cells and tissues. It is an important tool in genetics and molecular biology research, allowing scientists to investigate gene expression patterns and identify mutations or genetic abnormalities. Tissue sections are required for in-situ hybridization as they provide a thin and flat sample that can be easily hybridized with the nucleic acid probes. Without tissue sections, it would be difficult to identify and locate specific cells and tissues in a complex sample, making it challenging to study gene expression patterns and genetic abnormalities.
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Researchers have used the Compresstome® in their procedure to section mouse embryo hypothalamus that has been injected with DNA and electroporated. This procedure demonstrates how it is possible to transfect nuclei in the hypothalamus region which are less accessible than those in superficial regions. Following this procedure additional experiments can be performed such as immunohistochemistry and in situ hybridization.
Explore how scientists use the Compresstome® vibrating microtome to create tissue slices that combine lipophilic dye tracing, whole mount in situ hybridization, immunohistochemistry, and histology to extract the maximal possible amount of data.
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Mastorakos P, Mihelson N, Luby M, Burks SR, Johnson K, Hsia AW, Witko J, Frank JA, Latour L, McGavern DB. Temporally distinct myeloid cell responses mediate damage and repair after cerebrovascular injury. Nat Neurosci. 2021 Feb;24(2):245-258. Epub 2021 Jan 18. PMID: 33462481; PMCID: PMC7854523. Download PDF
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