In-situ hybridization

In-situ hybridization is a technique that uses nucleic acid probes to detect specific DNA or RNA sequences within cells and tissues. It is an important tool in genetics and molecular biology research, allowing scientists to investigate gene expression patterns and identify mutations or genetic abnormalities. Tissue sections are required for in-situ hybridization as they provide a thin and flat sample that can be easily hybridized with the nucleic acid probes. Without tissue sections, it would be difficult to identify and locate specific cells and tissues in a complex sample, making it challenging to study gene expression patterns and genetic abnormalities.

Recommended Models

VF-510-0Z

Compresstome vibrating microtome

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RF-1000

Rotary microtome

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Real lab examples

Genetic Manipulation of the Mouse Developing Hypothalamus Through In Utero Electroporation

Researchers have used the Compresstome^® in their procedure to section mouse embryo hypothalamus that has been injected with DNA and electroporated. This procedure demonstrates how it is possible to transfect nuclei in the hypothalamus region which are less accessible than those in superficial regions. Following this procedure additional experiments can be performed such as immunohistochemistry and in situ hybridization.

Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

Explore how scientists use the Compresstome^® vibrating microtome to create tissue slices that combine lipophilic dye tracing, whole mount in situ hybridization, immunohistochemistry, and histology to extract the maximal possible amount of data.

References

Chen X, Wolfe DA, Bindu DS, Zhang M, Taskin N, Goertsen D, Shay TF, Sullivan EE, Huang SF, Ravindra Kumar S, Arokiaraj CM, Plattner VM, Campos LJ, Mich JK, Monet D, Ngo V, Ding X, Omstead V, Weed N, Bishaw Y, Gore BB, Lein ES, Akrami A, Miller C, Levi BP, Keller A, Ting JT, Fox AS, Eroglu C, Gradinaru V. Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates. Nat Commun. 2023 Jun 8;14(1):3345. PMID: 37291094; PMCID: PMC10250345. Download PDF

Mastorakos P, Mihelson N, Luby M, Burks SR, Johnson K, Hsia AW, Witko J, Frank JA, Latour L, McGavern DB. Temporally distinct myeloid cell responses mediate damage and repair after cerebrovascular injury. Nat Neurosci. 2021 Feb;24(2):245-258. Epub 2021 Jan 18. PMID: 33462481; PMCID: PMC7854523. Download PDF

Rindner DJ, Proddutur A, Lur G. Cell-type-specific integration of feedforward and feedback synaptic inputs in the posterior parietal cortex. Neuron. 2022 Nov 16;110(22):3760-3773.e5. Epub 2022 Sep 9. PMID: 36087582; PMCID: PMC9671855. Download PDF

Full references list

Protocol

617-682-0586

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