Advantages of the Compresstome®

  • Minimal tissue damage: Compression stabilizes the lipid and various tissue types, holding the blood vessels in place, allowing a smooth section.
  • Smooth sections: tissue stabilization = No artifacts

Problems with traditional vibrating microtomes

  • High lipid content: Liver tissue contains a high amount of lipids, which can interfere with the vibratome cutting process and produce poor-quality sections. The lipids can cause the tissue to tear or smear, which can make it difficult to obtain usable sections.
  • Variability in tissue density: Liver tissue is composed of multiple cell types with different densities, which can make it challenging to produce uniform sections. The dense regions of the liver may cause the vibratome blade to skip or produce uneven section thickness.
  • Vascular structures: The liver is a highly vascular organ, which can make it difficult to obtain sections that are free of blood vessels. Blood vessels can cause the tissue to move during sectioning, leading to distorted or damaged sections.

Recommended Models

VF-510-0Z

Compresstome vibrating microtome

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Not sure which model is right for your needs?

Real lab examples

Making Precision-Cut Tissue Slices for Ex Vivo Assay Services

Visikol is a contract research services company focused on leveraging advanced imaging, 3D cell culture assays and digital pathology to accelerate the drug discovery and development process. In this webinar, Visikol explains the need for in vitro liver models to study livery injury. They demonstrate the standard assay format for creating precision-cut liver slices (PCLS), and explain how the Compresstome® VF-310-0Z vibrating microtome helps create uniform tissue slices that can be meaningfully compared between treatments. Visikol goes through how to use the Compresstome® step-by-step for making PCLS.

References

Cautivo KM, Matatia PR, Lizama CO, Mroz NM, Dahlgren MW, Yu X, Sbierski-Kind J, Taruselli MT, Brooks JF, Wade-Vallance A, Caryotakis SE, Chang AA, Liang HE, Zikherman J, Locksley RM, Molofsky AB. Interferon gamma constrains type 2 lymphocyte niche boundaries during mixed inflammation. Immunity. 2022 Feb 8;55(2):254-271.e7. PMID: 35139352; PMCID: PMC8852844. Download PDF

Liu P, Anandhan A, Chen J, Shakya A, Dodson M, Ooi A, Chapman E, White E, Garcia JG, Zhang DD. Decreased autophagosome biogenesis, reduced NRF2, and enhanced ferroptotic cell death are underlying molecular mechanisms of non-alcoholic fatty liver disease. Redox Biol. 2023 Feb;59:102570. Epub 2022 Dec 5. PMID: 36495698; PMCID: PMC9731892. Download PDF

Parlakgül G, Arruda AP, Pang S, Cagampan E, Min N, Güney E, Lee GY, Inouye K, Hess HF, Xu CS, Hotamışlıgil GS. Regulation of liver subcellular architecture controls metabolic homeostasis. Nature. 2022 Mar;603(7902):736-742. Epub 2022 Mar 9. PMID: 35264794; PMCID: PMC9014868. Download PDF

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