The lung is a vital organ responsible for oxygen uptake and removal of carbon dioxide from the body. It is essential for maintaining normal bodily function and supporting metabolism. Tissue sections are needed to study the lung as they provide a thin and flat sample that can be easily sliced and observed under a microscope. This allows researchers to investigate the cellular and molecular mechanisms underlying various lung functions, such as gas exchange, immune response, and lung development, and to identify changes that occur in lung diseases and disorders, such as asthma, chronic obstructive pulmonary disease (COPD), and lung cancer.
The Compresstome® is the most widely used tissue slicer in the United States for making precision-cut lung slices, providing healthier tissue for studies of respiratory physiology. For studies of lung anatomy and microstructures, the Compresstome® tissue slicer can section lung tissue as thin as 4µm. Fixed lung slices can then be processed for immunohistochemistry (IHC), in-situ hybridization (ISH), or H&E staining.
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Dr Astero Klampatsa (PhD) is a Team Leader in Cancer Immunotherapy at the Institute of Cancer Research, London, UK and a Senior Lecturer in King’s College London, UK. She focuses on developing novel CAR T cell therapies for mesothelioma and lung cancer, as well as the immunobiology of these malignancies for identification of markers of response to immunotherapy. In this webinar, Dr. Klampatsa will discuss how the Compresstome® was used to create precision-cut tumor slices (PCTS) as an ex vivo model for immunotherapy research.
The Compresstome® has been widely used by researchers worldwide for making precision-cut lung slices (PCLS). The Compresstome® uses agarose embedding prior to slicing to allow for the preservation of open alveoli and better tissue compliance. The video above shows Compresstome® sectioning PCLS for immunostaining to visualize the localization of various immune cell types in the lung. This protocol can be extended to visualize the location and function of many different cell types under a variety of conditions.
Dr. Tsilingiri is working on tumor immunotherapy and using the Compresstome vibrating microtome to examine the interaction between tumor tissues and autologous lymph node cells in slice cultures. This work is being carried out in the frame of an EU-funded Consortium, Tumour-LNoC (Tumour-Lymph node on a chip), with the ultimate goal of mimicking the metastatic process on a chip and monitor metastasizing cells in real time.
Dr. Koziol-White showcased the versatility of the precision cut lung slice system that she has developed and utilized to study airway function for almost two decades.
Dr. Claudia Loebel’s research involves the development of PCLS to probe mechanisms of early epithelial cell differentiation during lung injury and fibrosis.
Dr. Chioccioli:
Kim JH, Schaible N, Hall JK, Bartolák-Suki E, Deng Y, Herrmann J, Sonnenberg A, Behrsing HP, Lutchen KR, Krishnan R, Suki B. Multiscale stiffness of human emphysematous precision cut lung slices. Sci Adv. 2023 May 19;9(20):eadf2535. Epub 2023 May 19. PMID: 37205750; PMCID: PMC10198632. Download PDF
Lam M, Lamanna E, Organ L, Donovan C, Bourke JE. Perspectives on precision cut lung slices-powerful tools for investigation of mechanisms and therapeutic targets in lung diseases. Front Pharmacol. 2023 May 16;14:1162889. PMID: 37261291; PMCID: PMC10228656. Download PDF
Kim SY, Mongey R, Griffiths M, Hind M, Dean CH. An Ex Vivo Acid Injury and Repair (AIR) Model Using Precision-Cut Lung Slices to Understand Lung Injury and Repair. Curr Protoc Mouse Biol. 2020 Dec;10(4):e85. doi: 10.1002/cpmo.85. PMID: 33217226. Download PDF
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