Here are recommended speed and oscillation settings for tissue sectioning
These are suggested slicing parameters for the Compresstome® vibrating microtomes. Please note that you may need to adjust these settings to optimize tissue sectioning for your own experiments.
Tissue or Sample | Advance (Speed) | Oscillation (Frequency) | Suggested Agarose Concentration (%) |
---|---|---|---|
Lung
| 2-3 | 3-4 | 2% |
General fixed tissues | 1.5-2 | 3-4 | 2% |
Brain (live)
| 15.-2 | 3-4 | 1.8-2% |
Organotypic slices:
| 2-3 | 4-5 | 2% |
Retina (fixed or acute slices
| 1.5-2 | 5-6 | 2.5% |
Kidney (acute slices) | 3-4 | 5-6 | 2.5% |
Liver (acute slices) | 3-4 | 5-6 | 2.5% |
Cardiac (fixed or acute slices
| 1.5-2 | 5-6 | 2.5% |
Gut (fixed or acute slices) | 3-4 | 3-4 | 2.5% |
Plants (leaves and stems) | 3-4 | 4-5 | 2% |
Plants (seeds) | 6-8 | 2-3 | 2.5% |
Skin (epidermis) | 2-3 | 5-6 | 2.5% |
Muscle (fixed or acute slices) | 2-3 | 4-6 | 2.5% |
Adipose (fixed or acute slices) | 4-5 | 5-6 | 1.8% |
Lymph node (fixed or acute slices) | 1.5-2 | 5-6 | 2.5% |
Spleen (fixed or acute slices) | 3-4 | 6-7 | 2% |
Polymers or hard materials (for paint analysis, matrices, etc) | We recommend that you use a rotary microtome for hard material sectioning. Check out our product line of high quality rotary microtomes here. |
Below are speed and oscillation (frequency) conversions for Compresstome® VF-510-0Z vibratomes. The graphs help identify the linear speed (mm/s) and oscillation frequency (Hz) of the control box parameters.
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