Using the Compresstome in Plant Research

Plant Tissue for Research

Today, let’s dive into the world of plant tissue research! Did you know that methods and techniques for studying plant and animal tissue are often similar? To examine proteins on the microscopic level, processes to get tissue slices include dehydration, fixation, and embedding for immunohistochemical analyses. But sometimes, these steps are not suitable or are too harsh. In such cases, fresh plant tissue is needed.

Compresstome in Plant Research

The old fashioned method of free-hand sectioning of plant tissue has been performed for a very long time. Free-hand sectioning is done with razor blades, but this is difficult to apply to plant samples that are hard to hold or manipulate. Vibratomes or vibrating microtomes can be an excellent tool for cutting plant tissue sections, and these machines help generate high quality thin sections without the need to fix or freeze plant samples.

In this book chapter publication by Drs. Mohamed M. Mira, Edward C. Yeung, and Claudio Stasolla, the team describes a simple guide to use for cutting plant slices with the Compresstome for research. Here, they summarize how to use the Compresstome vibrating microtome to embed a plant sample:

Compresstome in Plant Research

Next, they are able to cut thin fresh plant tissue slices, which are then placed onto a glass slide for further experimental treatments. They are able to then visualize proteins as GFP signals in samples like corn roots.

Compresstome in Plant Research

They remark that:

“The use of the vibratome is convenient; it does not require special blades. Most importantly, it is effective on fresh tissues without pretreatment, such as fixation and dehydration that might cause artifacts typical of cryo- or paraffin-embedded sections. Vibratome sections preserve good structural and ultrastructural characteristics, and adjustments to the vibrating razor blades in terms of speed and vibration amplitude permit the user to optimize sectioning on diverse types of specimens.”

Share
Share on facebook
Share on twitter
Share on email
Share on pinterest
Share on print
Related Posts
Slicing the spinal cord part 1 and 2
Compresstome Vibrating Microtomes

Slicing the Spinal Cord

How do I embed and cut great spinal cord slice? You asked, and we listened! We realized that embedding spinal cord tissue for cutting slices

Precisionary Rotary Microtomes RF-600
Rotary Microtomes

Precisionary Rotary Microtomes in Action Videos!

Precisionary Rotary Microtomes in Action! We love helping scientists and clinicians get high quality tissue slices. With our new rotary microtome line, the best way to

Compresstome VF-800-0Z Cerebellum Primate Brain
Compresstome Vibrating Microtomes

Meet Our Compresstome VF-800-0Z!

Most basic science researchers use mice or rats as their animal model of choice. But what if you study larger animal models like guinea pigs,

Call Now Button617-682-0586