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Tissue Sectioning Solutions for Organotypic Slice Culture

Create healthy, viable tissue slices for organotypic slice cultures with Precisionary Instruments’ Compresstome vibratome, designed to maintain tissue integrity for long-term culture studies.

Get Healthy Tissues Slices Perfect for Organotypic Slice Culture Experiments

Organotypic cultured slices preserve the 3D cytoarchitecture of cell populations and offer a versatile system for short- and long-term experimental manipulations. This makes them especially valuable in physiology research, including brain slice cultures for neuroscience.

Since organotypic slice cultures require tissue slices to be incubated and kept alive outside the animal model, cell survival is critical. For brain slices, neuron viability is key for patch-clamp electrophysiology. These slices need a high ratio of viable neurons in the top cell layers that can be easily patch-clamped. To prevent neuron death, the cutting process must be rapid, using a vibratome that can quickly create high-quality brain slices without surface shearing.

Compresstome® VF-510-0Z

Rapid Sectioning for Viable Organotypic Slice Cultures

The Compresstome® VF-510-0Z is ideal for producing thin, precise tissue sections while preserving the viability and health of samples, making it the perfect tool for organotypic slice culture. Its fully automated system ensures consistent slices that maintain the physiological integrity of tissues, supporting long-term culture studies in areas such as neurobiology, toxicology, and drug testing. Experimental applications include:

With a 5-year warranty, the VF-510-0Z offers unmatched precision and ensures the viability of tissue slices, providing reliable support for organotypic culture research.

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Real Labs Trust Precisionary Vibratomes for Their Organotypic Slice Culture Experiments

Compresstome® for sectioning live myocardial slices for cardiac research

The Smyth Laboratory, led by James Smyth, Ph.D., studies cardiomyopathy at a subcellular level, searching for potential targets for therapeutic interventions to help restore normal cardiac function to diseased hearts. Here, Dr. Smyth shows how to section live myocardial slices with the Compresstome®, and uses them for tissue culture and calcium imaging.

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Using the Compresstome® in Immunotherapy Research

Dr Astero Klampatsa (PhD) is a Team Leader in Cancer Immunotherapy at the Institute of Cancer Research, London, UK and a Senior Lecturer in King’s College London, UK. She focuses on developing novel CAR T cell therapies for mesothelioma and lung cancer, as well as the immunobiology of these malignancies for identification of markers of response to immunotherapy. In this webinar, Dr. Klampatsa will discuss how the Compresstome® was used to create precision-cut tumor slices (PCTS) as an ex vivo model for immunotherapy research.

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Making Precision-Cut Tissue Slices for Ex Vivo Assay Services

Visikol is a contract research services company focused on leveraging advanced imaging, 3D cell culture assays and digital pathology to accelerate the drug discovery and development process. In this webinar, Visikol explains the need for in vitro liver models to study livery injury. They demonstrate the standard assay format for creating precision-cut liver slices (PCLS), and explain how the Compresstome® VF-310-0Z vibrating microtome helps create uniform tissue slices that can be meaningfully compared between treatments. Visikol goes through how to use the Compresstome® step-by-step for making PCLS.

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Slicing up the tumor: Lessons from attempted lung tumor slice cultures

Dr. Tsilingiri is working on tumor immunotherapy and using the Compresstome vibrating microtome to examine the interaction between tumor tissues and autologous lymph node cells in slice cultures. This work is being carried out in the frame of an EU-funded Consortium, Tumour-LNoC (Tumour-Lymph node on a chip), with the ultimate goal of mimicking the metastatic process on a chip and monitor metastasizing cells in real time.

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Precision cut lung slices (PCLS): A novel ex vivo model to study lung disease

Dr. Koziol-White showcased the versatility of the precision cut lung slice system that she has developed and utilized to study airway function for almost two decades.

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Empowering Neuroscience Research at University Hospital Würzburg with the Compresstome® Vibratome

At University Hospital Würzburg, researchers are using the Compresstome® vibrating microtome to advance neuroscience studies by creating high-quality brain tissue slices. This precision tool enables the team to perform detailed electrophysiology and imaging experiments, providing invaluable insights into brain function and contributing to cutting-edge discoveries in neurological health and disease.

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References

Gavert N, Zwang Y, Weiser R, Greenberg O, Halperin S, Jacobi O, Mallel G, Sandler O, Berger AJ, Stossel E, Rotin D, Grinshpun A, Kamer I, Bar J, Pines G, Saidian D, Bar I, Golan S, Rosenbaum E, Nadu A, Ben-Ami E, Weitzen R, Nechushtan H, Golan T, Brenner B, Nissan A, Margalit O, Hershkovitz D, Lahat G, Straussman R. Ex vivo organotypic cultures for synergistic therapy prioritization identify patient-specific responses to combined MEK and Src inhibition in colorectal cancer. Nat Cancer. 2022 Feb;3(2):219-231. Epub 2022 Feb 10. PMID: 35145327.

Greier MDC, Runge A, Dudas J, Carpentari L, Schartinger VH, Randhawa A, Mayr M, Petersson M, Riechelmann H. Optimizing culturing conditions in patient derived 3D primary slice cultures of head and neck cancer. Front Oncol. 2023 Mar 30;13:1145817. PMID: 37064104; PMCID: PMC10101142.

Ruiz-Garcia H, Zarco N, Watanabe F, De Araujo Farias V, Suarez-Meade P, Guerrero-Cazares H, Imitola J, Quinones-Hinojosa A, Trifiletti D. Development of Experimental Three-Dimensional Tumor Models to Study Glioblastoma Cancer Stem Cells and Tumor Microenvironment. Methods Mol Biol. 2023;2572:117-127. PMID: 36161412.

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Tissue Sectioning Solutions for Organotypic Slice Culture

Get Healthy Tissues Slices Perfect for Organotypic Slice Culture Experiments

Rapid Sectioning for Viable Organotypic Slice Cultures

Real Labs Trust Precisionary Vibratomes for Their Organotypic Slice Culture Experiments

Compresstome® for sectioning live myocardial slices for cardiac research

Using the Compresstome® in Immunotherapy Research

Making Precision-Cut Tissue Slices for Ex Vivo Assay Services

Slicing up the tumor: Lessons from attempted lung tumor slice cultures

Precision cut lung slices (PCLS): A novel ex vivo model to study lung disease

Empowering Neuroscience Research at University Hospital Würzburg with the Compresstome® Vibratome

References

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