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Precisionary Instruments offers a full line of high quality rotary microtomes for histopathology, research, and clinical purposes. Our microtomes give you reliable sections over a range of tissue slice thicknesses.
The Compresstome® is the most widely used tissue slicer in the United States for making precision-cut lung slices, providing healthier tissue for studies of respiratory physiology. For studies of lung anatomy and microstructures, the Compresstome® tissue slicer can section lung tissue as thin as 4µm. Fixed lung slices can then be processed for immunohistochemistry (IHC), in-situ hybridization (ISH), or H&E staining.
Not sure which model is right for your needs?
Dr Astero Klampatsa (PhD) is a Team Leader in Cancer Immunotherapy at the Institute of Cancer Research, London, UK and a Senior Lecturer in King’s College London, UK. She focuses on developing novel CAR T cell therapies for mesothelioma and lung cancer, as well as the immunobiology of these malignancies for identification of markers of response to immunotherapy. In this webinar, Dr. Klampatsa will discuss how the Compresstome® was used to create precision-cut tumor slices (PCTS) as an ex vivo model for immunotherapy research.
The Compresstome® has been widely used by researchers worldwide for making precision-cut lung slices (PCLS). The Compresstome® uses agarose embedding prior to slicing to allow for the preservation of open alveoli and better tissue compliance. The video above shows Compresstome® sectioning PCLS for immunostaining to visualize the localization of various immune cell types in the lung. This protocol can be extended to visualize the location and function of many different cell types under a variety of conditions.
Dr. Tsilingiri is working on tumor immunotherapy and using the Compresstome vibrating microtome to examine the interaction between tumor tissues and autologous lymph node cells in slice cultures. This work is being carried out in the frame of an EU-funded Consortium, Tumour-LNoC (Tumour-Lymph node on a chip), with the ultimate goal of mimicking the metastatic process on a chip and monitor metastasizing cells in real time.
Dr. Chioccioli:
Ghosh A, Koziol-White CJ, Asosingh K, Cheng G, Ruple L, Groneberg D, Friebe A, Comhair SA, Stasch JP, Panettieri RA Jr, Aronica MA, Erzurum SC, Stuehr DJ. Soluble guanylate cyclase as an alternative target for bronchodilator therapy in asthma. Proc Natl Acad Sci U S A. 2016 Apr 26;113(17):E2355-62. PMID: 27071111; PMCID: PMC4855555. Download PDF
Koziol-White CJ, Jia Y, Baltus GA, Cooper PR, Zaller DM, Crackower MA, Sirkowski EE, Smock S, Northrup AB, Himes BE, Alves SE, Panettieri RA Jr. Inhibition of spleen tyrosine kinase attenuates IgE-mediated airway contraction and mediator release in human precision cut lung slices. Br J Pharmacol. 2016 Nov;173(21):3080-3087. PMID: 27417329; PMCID: PMC5056227. Download PDF
Koziol-White CJ, Yoo EJ, Cao G, Zhang J, Papanikolaou E, Pushkarsky I, Andrews A, Himes BE, Damoiseaux RD, Liggett SB, Di Carlo D, Kurten RC, Panettieri RA Jr. Inhibition of PI3K promotes dilation of human small airways in a rho kinase-dependent manner. Br J Pharmacol. 2016 Sep;173(18):2726-38. PMID: 27352269; PMCID: PMC4995285. Download PDF
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Experimental protocols
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Contact us
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Contact us
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info@precisionary.com
Phone: 617-682-0586
Fax: 1-866-424-2216
© 2023 copyright
Privacy Policy