Original webinar date: March 1, 2022

Have you wondered how one mouse brain may be used for multiple experiments? Come discover the strategy behind using animal tissues for multi-use research experiments, so that your tissue samples can go further.

Dr. Yiying Zhang from Harvard Medical School and Massachusetts General Hospital is our guest webinar speaker.

Dr. Yiying Zhang has been studying neuropathogenesis, anesthesia neurotoxicity, anesthesia/surgery toxicity, postoperative neurocognitive disorder and postoperative delirium (POD) in Alzheimer’s disease (AD) since 2009. Specifically, she has studied the effects of anesthetics on mitochondrial function in vitro and in vivo, as well as the potential association between neuroinflammation and mitochondrial function following the anesthesia and/or surgery. Recently, Dr. Zhang has focused on studying potential gut microbiota-neuroinflammation-mitochondria cascade in the pathogenesis of POD. For this Precisionary webinar, Dr. Zhang will discuss a “3D” use of animal tissues in planning experimental designs in academic research.

Original webinar date: July 6, 2021

Dr. Henderson is an Assistant Professor in the Department of Biomedical Sciences at Marshall University’s Joan C. Edwards School of Medicine. He received a BS (with distinction) in Chemistry at The Ohio State University, where he started research, initially as an Analytical Chemist in the lab of Dr. John Olesik. He went on to receive his PhD in Pharmacology at The Ohio State University in the lab of Dr. Dennis McKay. Afterwards, his professional journey continued his development as a NIDA-NIH Postdoctoral Fellow at the California Institute of Technology in the lab of Dr. Henry Lester. Before starting his faculty position at Marshall University, Dr. Henderson also trained as a visiting postdoctoral fellow at Yale University (under Dr Nii Addy and Dr. Marina Picciotto).

In addition to his responsibilities at Marshall University, Dr. Henderson is now one of two co-Chairs for the Basic Science Network in the Society for Research on Nicotine and Tobacco (www.SRNT.org).

The Henderson lab focuses on the role tobacco and vaping flavors play in addiction-related behaviors. Thus far, we have shown that menthol and green apple flavors can enhance nicotine vapor self-administration and do so by directly altering dopamine neurons in the midbrain.

Original webinar date: March 22, 2021

Jonathan T. Ting is an Assistant Investigator at the Allen Institute, where he joined in 2013 to provide electrophysiology expertise for the Human Cell Types program, and to develop functional assays on human ex vivo brain slides. Dr. Ting has more than 15 years of experience in patch clamp electrophysiology. He studied the neural circuitry basis of psychiatric disorders during his postdoctoral fellowships at Duke University and the McGovern Institute for Brain Research at MIT, and he developed and characterized several transgenic mouse lines now widely employed for nervous system research involving optogenetics.

For this webinar, Dr. Ting provides reflections of his experience on a decade of patching adult brain slices. He will:

    • Discuss which key steps in the brain slice process is most important and why
    • Challenge our conventional beliefs of slicing solutions and methodologies
    • Recommend tips and tricks based on his experience and research

Original webinar date: November 2, 2020

Webinar objectives:

    • To understand frozen section technique, when it is used, and its applications in research and clinical studies.
    • To learn how to cut gross specimens of tissue to prepare for cutting on the cryostat.
    • To learn how to section tissue on the cryostat step-by-step:
      • How to embed tissue in OCT
      • How to use a heat extractor during embedding
      • How to section thin slices on the cryostat and collect slices onto glass slides
    • To learn about the basic dyes used in pathology (H&E)
    • To understand how to troubleshoot:
      • Technical issues
      • Staining issues
      • Cutting artifacts (under/over-freezing, knife artifacts, ice crystals, air bubbles)
      • Tissue types (small/large, fatty tissue, hard tissue)

Original webinar date: September 3, 2020

Webinar objectives:

    • To understand and appreciate why tissue slices are used in research, and what slices are used for experimentally.
    • To understand how the common fundamental cutting parameters affect tissue slice quality and health, and learn about possible experimental solutions.
    • To explore and share tips & tricks during the cutting process to improve tissue slice quality.