Liver

Problems with traditional vibrating microtomes

• High lipid content: Liver tissue contains a high amount of lipids, which can interfere with the vibratome cutting process and produce poor-quality sections. The lipids can cause the tissue to tear or smear, which can make it difficult to obtain usable sections.
• Variability in tissue density: Liver tissue is composed of multiple cell types with different densities, which can make it challenging to produce uniform sections. The dense regions of the liver may cause the vibratome blade to skip or produce uneven section thickness.
• Vascular structures: The liver is a highly vascular organ, which can make it difficult to obtain sections that are free of blood vessels. Blood vessels can cause the tissue to move during sectioning, leading to distorted or damaged sections.