Get A Quote

In-situ hybridization

In-situ hybridization is a technique that uses nucleic acid probes to detect specific DNA or RNA sequences within cells and tissues. It is an important tool in genetics and molecular biology research, allowing scientists to investigate gene expression patterns and identify mutations or genetic abnormalities. Tissue sections are required for in-situ hybridization as they provide a thin and flat sample that can be easily hybridized with the nucleic acid probes. Without tissue sections, it would be difficult to identify and locate specific cells and tissues in a complex sample, making it challenging to study gene expression patterns and genetic abnormalities.

Recommended Model/s

VF-510-0Z

Compresstome vibrating microtome

Learn more

RF-1000

Rotary microtome

Learn more

Not sure which model is right for your needs?

Real lab examples

Play Video

Genetic Manipulation of the Mouse Developing Hypothalamus Through In Utero Electroporation

Researchers have used the Compresstome® in their procedure to section mouse embryo hypothalamus that has been injected with DNA and electroporated. This procedure demonstrates how it is possible to transfect nuclei in the hypothalamus region which are less accessible than those in superficial regions. Following this procedure additional experiments can be performed such as immunohistochemistry and in situ hybridization.

Watch
Download
Play Video

Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

Explore how scientists use the Compresstome® vibrating microtome to create tissue slices that combine lipophilic dye tracing, whole mount in situ hybridization, immunohistochemistry, and histology to extract the maximal possible amount of data.

Watch
Download

References

Haddad-Tóvolli R, Heide M, Zhou X, Blaess S, Alvarez-Bolado G. Mouse thalamic differentiation: gli-dependent pattern and gli-independent prepattern. Front Neurosci. 2012 Feb 22;6:27. PMID: 22371696; PMCID: PMC3283895. Download PDF

Li S, Folkvord JM, Rakasz EG, Abdelaal HM, Wagstaff RK, Kovacs KJ, Kim HO, Sawahata R, MaWhinney S, Masopust D, Connick E, Skinner PJ. Simian Immunodeficiency Virus-Producing Cells in Follicles Are Partially Suppressed by CD8+ Cells In Vivo. J Virol. 2016 Nov 28;90(24):11168-11180. PMID: 27707919; PMCID: PMC5126374. Download PDF

Mylvaganam GH, Rios D, Abdelaal HM, Iyer S, Tharp G, Mavinger M, Hicks S, Chahroudi A, Ahmed R, Bosinger SE, Williams IR, Skinner PJ, Velu V, Amara RR. Dynamics of SIV-specific CXCR5+ CD8 T cells during chronic SIV infection. Proc Natl Acad Sci U S A. 2017 Feb 21;114(8):1976-1981. PMID: 28159893; PMCID: PMC5338410. Download PDF

See more

Full references list

Protocol

Transform your research process

You’ll hear back from us in one business day

info@precisionary.com

Phone: 617-682-0586

Fax: 1-866-424-2216

Our products

Compresstome Vibrating Microtomes

Rotary Microtomes

Help & support

Ordering guidelines

Warranty and service plans

Repair support

Experimental protocols

Partnerships and Distributors

Ordering guidelines

Warranty and service plans

Repair support

Experimental protocols

Partnerships and Distributors

Videos

User manuals

FAQs

Contact us

Video

User manuals

FAQs

Contact us

Facebook-f Twitter Youtube Linkedin-in

© 2023 copyright

Privacy Policy

Contact us

info@precisionary.com

Phone: 617-682-0586

Fax: 1-866-424-2216

© 2023 copyright

Privacy Policy

Facebook-f Twitter Youtube Linkedin-in
Call Now Button617-682-0586