APPLICATIONS | EXPERIMENTS

Tissue Sectioning Solutions for Precision-cut Tissue Slices (PCTS)

Create healthy, viable precision-cut tissue slices for drug screening, toxicology, and metabolism studies using Precisionary Instruments’ Compresstome VF-510-0Z.

High-Quality Precision-Cut Tissue Slices for Drug Screening and Toxicology

Precision-cut tissue slices (PCTS) are essential for studying organ responses to drugs and toxic compounds, as well as for metabolism and therapeutic development studies. These slices must remain viable and structurally intact to ensure accurate data in ex vivo models.

Precisionary Instruments’ Compresstome VF-510-0Z vibratome is designed to create thin, precise, and healthy tissue slices from multiple organs, including precision-cut liver slices (PCLS) and precision-cut kidney slices (PCKS). This enables researchers to perform drug screening, toxicology studies, and metabolic assays with reliable and reproducible results, providing critical insights into organ function and therapeutic efficacy.

Compresstome® VF-510-0Z

Precision Vibrating Microtome for Precision-Cut Tissue Slices

The Compresstome® VF-510-0Z is engineered to produce high-quality, viable tissue slices from organs such as the liver, kidney, and lung. These slices are ideal for drug screening, therapeutics development, toxicology, and metabolism studies. The fully automated system creates thin, uniform sections while maintaining the physiological integrity of organ tissues, ensuring accurate results for research and clinical applications. Experimental applications include:

With a 5-year warranty, the VF-510-0Z ensures reliable performance for producing healthy tissue slices across a wide range of organ types, critical for advancing drug discovery and therapeutic development.

VIEW SPECS

Real Labs Trust Precisionary Vibratomes for their PCTS Experiments

Using the Compresstome® in Immunotherapy Research

Dr Astero Klampatsa (PhD) is a Team Leader in Cancer Immunotherapy at the Institute of Cancer Research, London, UK and a Senior Lecturer in King’s College London, UK. She focuses on developing novel CAR T cell therapies for mesothelioma and lung cancer, as well as the immunobiology of these malignancies for identification of markers of response to immunotherapy. In this webinar, Dr. Klampatsa will discuss how the Compresstome® was used to create precision-cut tumor slices (PCTS) as an ex vivo model for immunotherapy research.

WATCH NOW

Making Precision-Cut Tissue Slices for Ex Vivo Assay Services

Visikol is a contract research services company focused on leveraging advanced imaging, 3D cell culture assays and digital pathology to accelerate the drug discovery and development process. In this webinar, Visikol explains the need for in vitro liver models to study livery injury. They demonstrate the standard assay format for creating precision-cut liver slices (PCLS), and explain how the Compresstome® VF-310-0Z vibrating microtome helps create uniform tissue slices that can be meaningfully compared between treatments. Visikol goes through how to use the Compresstome® step-by-step for making PCLS.

WATCH NOW

Immunology and Infection: The Compresstome® for Precision-Cut Lung Slices

The Compresstome® has been widely used by researchers worldwide for making precision-cut lung slices (PCLS). The Compresstome® uses agarose embedding prior to slicing to allow for the preservation of open alveoli and better tissue compliance. This video shows Compresstome® sectioning PCLS for immunostaining to visualize the localization of various immune cell types in the lung. This protocol can be extended to visualize the location and function of many different cell types under a variety of conditions.

WATCH NOW

Slicing up the tumor: Lessons from attempted lung tumor slice cultures

Dr. Tsilingiri is working on tumor immunotherapy and using the Compresstome vibrating microtome to examine the interaction between tumor tissues and autologous lymph node cells in slice cultures. This work is being carried out in the frame of an EU-funded Consortium, Tumour-LNoC (Tumour-Lymph node on a chip), with the ultimate goal of mimicking the metastatic process on a chip and monitor metastasizing cells in real time.

WATCH NOW

Precision cut lung slices (PCLS): A novel ex vivo model to study lung disease

Dr. Koziol-White showcased the versatility of the precision cut lung slice system that she has developed and utilized to study airway function for almost two decades.

WATCH NOW

Precision cut lung slices (PCLS) for probing mechanisms of pulmonary fibrosis

Dr. Claudia Loebel’s research involves the development of PCLS to probe mechanisms of early epithelial cell differentiation during lung injury and fibrosis.

WATCH NOW

Spatiotemporal Coordination of Stem Cell Behavior Following Alveolar Injury

Described motility of alveolar stem cells as a new injury response mechanism in the lung and reveal properties of stem cell motility at high cellular resolution Explained early highly dynamic behavior of AT2 cells post injury, including migration within and between alveoli Characterized the emergence of at least three distinct morphokinetic AT2 cell states associated with AT2 stem cell injury response Shown how small molecule-based inhibition of Rho-associated protein kinase (ROCK) pathway significantly reduced motility of AT2 stem cells following injury and reduced expression of Krt8, a known marker of intermediate progenitor cells

WATCH NOW

References

Ko J, Wilkovitsch M, Oh J, Kohler RH, Bolli E, Pittet MJ, Vinegoni C, Sykes DB, Mikula H, Weissleder R, Carlson JCT. Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes. Nat Biotechnol. 2022 Nov;40(11):1654-1662. Epub 2022 Jun 2. PMID: 35654978; PMCID: PMC9669087.

Liu P, Dodson M, Li H, Schmidlin CJ, Shakya A, Wei Y, Garcia JGN, Chapman E, Kiela PR, Zhang QY, White E, Ding X, Ooi A, Zhang DD. Non-canonical NRF2 activation promotes a pro-diabetic shift in hepatic glucose metabolism. Mol Metab. 2021 Sep;51:101243. Epub 2021 Apr 30. PMID: 33933676; PMCID: PMC8164084.

Weidinger A, Dungel P, Perlinger M, Singer K, Ghebes C, Duvigneau JC, Müllebner A, Schäfer U, Redl H, Kozlov AV. Experimental data suggesting that inflammation mediated rat liver mitochondrial dysfunction results from secondary hypoxia rather than from direct effects of inflammatory mediators. Front Physiol. 2013 Jun 7;4:138. PMID: 23760194; PMCID: PMC3675332.

Request a

Quote

Take the first step to better tissue sectioning. Fill out the form to request a quote from Precisionary Instruments. We’ll respond within one business day.

Tissue Sectioning Solutions for Precision-cut Tissue Slices (PCTS)

High-Quality Precision-Cut Tissue Slices for Drug Screening and Toxicology

Precision Vibrating Microtome for Precision-Cut Tissue Slices

Real Labs Trust Precisionary Vibratomes for their PCTS Experiments

Using the Compresstome® in Immunotherapy Research

Making Precision-Cut Tissue Slices for Ex Vivo Assay Services

Immunology and Infection: The Compresstome® for Precision-Cut Lung Slices

Slicing up the tumor: Lessons from attempted lung tumor slice cultures

Precision cut lung slices (PCLS): A novel ex vivo model to study lung disease

Precision cut lung slices (PCLS) for probing mechanisms of pulmonary fibrosis

Spatiotemporal Coordination of Stem Cell Behavior Following Alveolar Injury

References

Request a

Quote

Company

Applications

Support

Products