Making healthy tissue slices with a vibratome is important for electrophysiology because it allows researchers to study the electrical activity of neurons in a controlled and precise manner. Healthy slices enable accurate recordings of neuronal responses and facilitate the analysis of synaptic transmission, ion channels, and membrane properties. The vibratome produces thin and consistent slices, which reduce variability in experimental data and enhance the reliability and reproducibility of electrophysiological studies.
Compresstome® produces brain slices with higher neuronal viability: This study demonstrates significantly higher proportions of live neurons in many brain regions from slices cut with a Compresstome® vibrating microtome, compared to other vibratomes.
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For over a decade, researchers at the Allen Institute for Brain Science — often heralded as leaders in the field — have been using the Compresstome® vibrating microtome to help give them better brain slices with increased longevity and reduced damage to surface neurons.
Jonathan T. Ting is an Assistant Investigator at the Allen Institute, where he joined in 2013 to provide electrophysiology expertise for the Human Cell Types program, and to develop functional assays on human ex vivo brain slides. In this webinar, Dr. Ting discusses which key steps in the brain slice process is most important and why, and challenges our conventional beliefs of slicing solutions and methodologies.
Assistant Professor in the Department of Biomedical Sciences at Marshall University’s Joan C. Edwards School of Medicine. In addition, Dr. Henderson and his focus on the role tobacco and vaping flavors play in addiction-related behaviors, and uses the Compresstome® vibrating microtome to make all of their acute brain slices for patch-clamp electrophysiology.
The Smyth Laboratory, led by James Smyth, Ph.D., studies cardiomyopathy at a subcellular level, searching for potential targets for therapeutic interventions to help restore normal cardiac function to diseased hearts. Here, Dr. Smyth shows how to section live myocardial slices with the Compresstome®, and uses them for tissue culture and calcium imaging.
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Li L, Durand-de Cuttoli R, Aubry AV, Burnett CJ, Cathomas F, Parise LF, Chan KL, Morel C, Yuan C, Shimo Y, Lin HY, Wang J, Russo SJ. Social trauma engages lateral septum circuitry to occlude social reward. Nature. 2023 Jan;613(7945):696-703. Epub 2022 Nov 30. PMID: 36450985; PMCID: PMC9876792. Download PDF
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