Histopathology is the study of tissues and cells under a microscope to identify disease or abnormal changes in the body. It is a crucial tool in the diagnosis and treatment of various diseases, including cancer. Histopathology requires tissue sections because it allows the pathologist to examine the tissue’s structure and cellular composition in detail, which provides valuable information about the disease process. Without tissue sections, it would be difficult to identify specific features or changes in the tissue that are characteristic of a particular disease.
Histopathology is the study of disease by using a microscope to examine tissues or surgical biopsies that are processed, fixed, sectioned, and plated onto glass slides. Tissue slices are then processed by staining, where different dyes and antibodies help visualize specific components of tissues and proteins. Hematoxylin-Eosin (H&E) staining has been used in pathology for over a century.
Because histopathology requires extremely fine and thin tissue sections (often down to 5µm or thinner), obtaining consistent sections is crucial for further processing. The process of cutting tissue slices also needs to occur rapidly because serial sections are needed. Therefore, histology and pathology require a rotary microtome that can quickly make high quality thin tissue slices without friction, chattermarks, or inconsistencies.
Not sure which model is right for your needs?
Our rotary microtome RF-1000 is a fully automated microtome. Check out its keys features, and see it cutting specimen samples in this short video.
Dr Astero Klampatsa (PhD) is a Team Leader in Cancer Immunotherapy at the Institute of Cancer Research, London, UK and a Senior Lecturer in King’s College London, UK. She focuses on developing novel CAR T cell therapies for mesothelioma and lung cancer, as well as the immunobiology of these malignancies for identification of markers of response to immunotherapy. In this webinar, Dr. Klampatsa will discuss how the Compresstome® was used to create precision-cut tumor slices (PCTS) as an ex vivo model for immunotherapy research.
Explore how scientists use the Compresstome® vibrating microtome to create tissue slices that combine lipophilic dye tracing, whole mount in situ hybridization, immunohistochemistry, and histology to extract the maximal possible amount of data.
Dr. Tsilingiri is working on tumor immunotherapy and using the Compresstome vibrating microtome to examine the interaction between tumor tissues and autologous lymph node cells in slice cultures. This work is being carried out in the frame of an EU-funded Consortium, Tumour-LNoC (Tumour-Lymph node on a chip), with the ultimate goal of mimicking the metastatic process on a chip and monitor metastasizing cells in real time.
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Mastorakos P, Mihelson N, Luby M, Burks SR, Johnson K, Hsia AW, Witko J, Frank JA, Latour L, McGavern DB. Temporally distinct myeloid cell responses mediate damage and repair after cerebrovascular injury. Nat Neurosci. 2021 Feb;24(2):245-258. Epub 2021 Jan 18. PMID: 33462481; PMCID: PMC7854523. Download PDF
Rindner DJ, Proddutur A, Lur G. Cell-type-specific integration of feedforward and feedback synaptic inputs in the posterior parietal cortex. Neuron. 2022 Nov 16;110(22):3760-3773.e5. Epub 2022 Sep 9. PMID: 36087582; PMCID: PMC9671855. Download PDF
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