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APPLICATIONS | EXPERIMENTS

Tissue Sectioning Solutions for In-Situ Hybridization

Achieve precise tissue sections for in-situ hybridization with Precisionary Instruments’ vibratomes, microtomes, and cryostats.

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Optimize In-Situ Hybridization with High-Quality Tissue Sections

In-situ hybridization (ISH) uses nucleic acid probes to detect specific DNA or RNA sequences in cells and tissues, making it a crucial technique in genetics and molecular biology. To effectively investigate gene expression patterns and identify genetic abnormalities, thin, flat tissue sections are essential for optimal probe hybridization. Precisionary Instruments’ vibratomes, microtomes, and cryostats produce consistent, reliable tissue sections that ensure accurate localization of nucleic acid sequences. Our products help researchers achieve high-quality results in ISH studies, enabling precise detection and analysis of gene expression and mutations.

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Compresstome® VF-510-0Z

Precise, Thin Sections for In-situ Hybridization

The Compresstome® VF-510-0Z is designed to produce ultra-thin, consistent tissue sections that are essential for nucleic acid probe hybridization in In-situ Hybridization. This fully automated system ensures reliable, artifact-free sectioning, critical for accurate gene expression studies. Experimental applications include:

  • Thin sectioning for ISH
  • Consistent, smooth slices for nucleic acid probe hybridization
  • Reliable sections for genetic and molecular biology research
  • Ability to make live tissue slices for genetic sequencing

With a 5-year warranty, the VF-510-0Z provides unmatched precision for In-situ Hybridization applications.

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RF 1000 Front Right Angle
RF 1000 Specimen Clamp
RF 1000 Knife Holder Set Knob
RF 1000 Knife Holder Cutting Stage
RF 1000 Front Left Angle
RF 1000 Front
RF 1000 Foot Press
RF 1000 Cutting Stage Front
RF 1000 Cutting Stage Front Left

Rotary Microtome RF-1000

Thin, Consistent Sections for In-situ Hybridization

The RF-1000 rotary microtome excels at producing ultra-thin, consistent sections ideal for nucleic acid hybridization in In-situ Hybridization. Its fully automated system ensures smooth, artifact-free slices, crucial for high-quality hybridization and molecular analysis. Experimental applications include:

  • Thin tissue sections for In-situ Hybridization
  • Consistent, ultra-thin slices for nucleic acid hybridization
  • Reliable sections for precise gene expression studies

With a 3-year warranty, the RF-1000 offers precision and consistency for all In-situ Hybridization tasks.

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Cryostat CF-6100

Consistent, Frozen Sections for In-situ Hybridization

The Precisionary Cryostat CF-6100 is built for producing thin, consistent frozen sections necessary for nucleic acid hybridization in In-situ Hybridization. Its automated features ensure reproducibility and preserve the integrity of the sample, ensuring accurate and efficient hybridization. Experimental applications include:

  • Frozen tissue sectioning for In-situ Hybridization
  • Consistent, thin sections for nucleic acid probe hybridization
  • Preservation of tissue integrity for accurate gene expression analysis

As a reliable workhorse, the CF-6100 ensures consistent frozen sectioning for In-situ Hybridization applications over time.

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Paraffin Dispenser Cold plate
Paraffin Dispenser spout
Paraffin Dispenser foot pedal
Paraffin Dispenser front left

TEC-200 Tissue Embedding Center

Efficient Tissue Processing for In-Situ Hybridization

The TEC-200 Tissue Embedding Center is designed for seamless embedding. It offers automation for precise and consistent results. Ideal for in-situ hybridization, its ergonomic design ensures operator comfort. Experience stability and precision cutting, and elevate your tissue processing with the TEC-200 from Precisionary Instruments.

  • Intuitive controls
  • Fully programmable ON/OFF control allows automatic system start and stop.
  • Large drain holes that allow easy drainage into trays under all working areas
  • Trimming plates on both sides, as well as a separate heated and cooling workstation, allow easy workflow
  • Simple maintenance and repair in case of a malfunction

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Real Labs Trust Precisionary Vibratomes for Their In-Situ Hybridization Applications

Genetic Manipulation of the Mouse Developing Hypothalamus Through In-utero Electroporation

Researchers have used the Compresstome® in their procedure to section mouse embryo hypothalamus that has been injected with DNA and electroporated. This procedure demonstrates how it is possible to transfect nuclei in the hypothalamus region which are less accessible than those in superficial regions. Following this procedure additional experiments can be performed such as immunohistochemistry and in situ hybridization.

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Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

Explore how scientists use the Compresstome® vibrating microtome to create tissue slices that combine lipophilic dye tracing, whole mount in situ hybridization, immunohistochemistry, and histology to extract the maximal possible amount of data.

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References

Chen X, Wolfe DA, Bindu DS, Zhang M, Taskin N, Goertsen D, Shay TF, Sullivan EE, Huang SF, Ravindra Kumar S, Arokiaraj CM, Plattner VM, Campos LJ, Mich JK, Monet D, Ngo V, Ding X, Omstead V, Weed N, Bishaw Y, Gore BB, Lein ES, Akrami A, Miller C, Levi BP, Keller A, Ting JT, Fox AS, Eroglu C, Gradinaru V. Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates. Nat Commun. 2023 Jun 8;14(1):3345. PMID: 37291094; PMCID: PMC10250345.

Mastorakos P, Mihelson N, Luby M, Burks SR, Johnson K, Hsia AW, Witko J, Frank JA, Latour L, McGavern DB. Temporally distinct myeloid cell responses mediate damage and repair after cerebrovascular injury. Nat Neurosci. 2021 Feb;24(2):245-258. Epub 2021 Jan 18. PMID: 33462481; PMCID: PMC7854523.

Rindner DJ, Proddutur A, Lur G. Cell-type-specific integration of feedforward and feedback synaptic inputs in the posterior parietal cortex. Neuron. 2022 Nov 16;110(22):3760-3773.e5. Epub 2022 Sep 9. PMID: 36087582; PMCID: PMC9671855.

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