APPLICATIONS | ANIMALS

Tissue Sectioning Solutions for Mouse Models

Create consistent mouse tissue slices for biomedical and neuroscience research using Precisionary Instruments’ vibratomes, microtomes, and cryostats.

Get High-Quality Mouse Tissue Slices

Mice are key models in biomedical research due to their genetic and physiological similarities to humans. They are widely used in studies of cancer, diabetes, neurological disorders, and preclinical drug testing.

Mouse brain slices are crucial for research in electrophysiology, optogenetics, and calcium imaging. The Compresstome® produces healthy brain slices with a greater number of live neurons, offering longer viability for neurological research.

In addition to brain tissue, mouse liver, heart, and lungs are also studied for disease modeling. The Compresstome® supports fresh and fixed tissue sectioning, making it ideal for organotypic cultures and histological studies.

Compresstome® VF-510-0Z

Precision Vibrating Microtome for Mouse Tissue Slices

The VF-510-0Z creates precise tissue slices for mouse brain and other organs. It supports both fresh and fixed tissue, ideal for neurological studies and histology.

Experimental Applications Include:

With a 5-year warranty, the VF-510-0Z ensures consistent and reliable tissue sectioning for long-term research.

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Rotary Microtome RF-1000

Fully Automated Microtome for Thin Mouse Tissue Sections

The RF-1000 provides precision for cutting thin tissue sections, especially from mouse organs like the brain and liver. It’s essential for immunohistochemistry and high-resolution imaging.

Experimental Applications Include:

With a 3-year warranty, the RF-1000 delivers consistent performance for mouse tissue research.

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Cryostat CF-6100

Cryostat for Frozen Sectioning of Mouse Tissues

The CF-6100 cryostat is designed for frozen sectioning of mouse tissues, especially brain tissue, ensuring the integrity of OCT-embedded samples. It’s ideal for producing frozen sections for immunohistochemistry and imaging.

Experimental Applications Include:

Acting as a laboratory workhorse, the CF-6100 ensures dependable frozen sectioning for mouse tissues in biomedical research

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TEC-200 Tissue Embedding Center

Efficient Tissue Processing for Mouse Models

The TEC-200 Tissue Embedding Center is designed for seamless embedding. It offers automation for precise and consistent results. Ideal for mouse models, its ergonomic design ensures operator comfort. Experience stability and precision cutting, and elevate your tissue processing with the TEC-200 from Precisionary Instruments.

Intuitive controls
Fully programmable ON/OFF control allows automatic system start and stop.
Large drain holes that allow easy drainage into trays under all working areas
Trimming plates on both sides, as well as a separate heated and cooling workstation, allow easy workflow
Simple maintenance and repair in case of a malfunction

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Real Labs Trust Precisionary Vibratome for Mouse Tissue Slicing

Reflections on a decade of patching in adult brain slices

Jonathan T. Ting is an Assistant Investigator at the Allen Institute, where he joined in 2013 to provide electrophysiology expertise for the Human Cell Types program, and to develop functional assays on human ex vivo brain slides. In this webinar, Dr. Ting discusses which key steps in the brain slice process is most important and why, and challenges our conventional beliefs of slicing solutions and methodologies.

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Using electrophysiological methods to examine e-cigarette flavors’ effect on dopamine neuron function

Assistant Professor in the Department of Biomedical Sciences at Marshall University’s Joan C. Edwards School of Medicine. In addition, Dr. Henderson and his focus on the role tobacco and vaping flavors play in addiction-related behaviors, and uses the Compresstome® vibrating microtome to make all of their acute brain slices for patch-clamp electrophysiology.

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Studying Cell Motility in Glioblastoma with Precision-Cut Brain Sections

Dr. Natanael Zarco, a Research Associate at Mayo Clinic, uses the Compresstome® vibratome to prepare precision-cut brain sections for studying glioblastoma cell migration. In this webinar, Dr. Zarco explores how these sections enable his research into how glioblastoma stem cells invade brain regions, providing critical insights into cancer progression and potential therapeutic targets.

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Using the Compresstome® in Immunotherapy Research

Dr Astero Klampatsa (PhD) is a Team Leader in Cancer Immunotherapy at the Institute of Cancer Research, London, UK and a Senior Lecturer in King’s College London, UK. She focuses on developing novel CAR T cell therapies for mesothelioma and lung cancer, as well as the immunobiology of these malignancies for identification of markers of response to immunotherapy. In this webinar, Dr. Klampatsa will discuss how the Compresstome® was used to create precision-cut tumor slices (PCTS) as an ex vivo model for immunotherapy research.

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Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method

Often heralded as leaders in the field, the Allen Brain institute performs pioneering research on all manner of brain tissue. Working with brain tissue can often be as frustrating as it is rewarding. Slicing brain tissue presents many challenges. The tissue is a combination of soft and fibrous regions. For over a decade, researchers at the Allen Institute for Brain Science have been using the Compresstome® vibrating microtome to help give them better brain slices with increased longevity and reduced damage to surface neurons. This enables neuroscientists to have healthy neurons for patch-clamp electrophysiology experiments.

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Genetic Manipulation of the Mouse Developing Hypothalamus Through In Utero Electroporation

Researchers have used the Compresstome® in their procedure to section mouse embryo hypothalamus that has been injected with DNA and electroporated. This procedure demonstrates how it is possible to transfect nuclei in the hypothalamus region which are less accessible than those in superficial regions. Following this procedure additional experiments can be performed such as immunohistochemistry and in situ hybridization.

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Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

Explore how scientists use the Compresstome® vibrating microtome to create tissue slices that combine lipophilic dye tracing, whole mount in situ hybridization, immunohistochemistry, and histology to extract the maximal possible amount of data.

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From two-dimensional to three-dimensional histopathology using a Compresstome®

Dr. Wong shares how he built a custom-made Compresstome® for high-speed histological 3D imaging of whole organs like brains.

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Interactions between Saturated Fat, Cytokines, and Microglia in the Ventral Striatum

Dr. Fordahl will highlight how proinflammatory cytokines alter dopamine terminal function, and how increasing dietary fat intake may enhance microglial activity.

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The locus coeruleus (LC) mediates behavioral flexibility

Dr. Greta Vargova is going to present their most recent publication, in which they show the link between locus coeruleus activity and behavioral flexibility.

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Investigating how sodium channels regulate the firing of cerebellar Purkinje neurons requires healthy brain slices

Dr. Ransdell explores how the Compresstome vibrating microtome is used to produce healthy brain slices for electrophysiology. He studies adult Purkinje neurons in mouse cerebellar brain slices.

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References

Godino A, Salery M, Durand-de Cuttoli R, Estill MS, Holt LM, Futamura R, Browne CJ, Mews P, Hamilton PJ, Neve RL, Shen L, Russo SJ, Nestler EJ. Transcriptional control of nucleus accumbens neuronal excitability by retinoid X receptor alpha tunes sensitivity to drug rewards. Neuron. 2023 May 3;111(9):1453-1467.e7. Epub 2023 Mar 7. PMID: 36889314; PMCID: PMC10164098.

Li L, Durand-de Cuttoli R, Aubry AV, Burnett CJ, Cathomas F, Parise LF, Chan KL, Morel C, Yuan C, Shimo Y, Lin HY, Wang J, Russo SJ. Social trauma engages lateral septum circuitry to occlude social reward. Nature. 2023 Jan;613(7945):696-703. Epub 2022 Nov 30. PMID: 36450985; PMCID: PMC9876792.

Wang C, Hyams B, Allen NC, Cautivo K, Monahan K, Zhou M, Dahlgren MW, Lizama CO, Matthay M, Wolters P, Molofsky AB, Peng T. Dysregulated lung stroma drives emphysema exacerbation by potentiating resident lymphocytes to suppress an epithelial stem cell reservoir. Immunity. 2023 Mar 14;56(3):576-591.e10. Epub 2023 Feb 22. PMID: 36822205.

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Bring greater precision and consistency to your mouse tissue slices. Fill out the form to request a quote from Precisionary Instruments. We’ll respond within one business day.

Tissue Sectioning Solutions for Mouse Models

Get High-Quality Mouse Tissue Slices

Precision Vibrating Microtome for Mouse Tissue Slices

Fully Automated Microtome for Thin Mouse Tissue Sections

Cryostat for Frozen Sectioning of Mouse Tissues

Efficient Tissue Processing for Mouse Models

Real Labs Trust Precisionary Vibratome for Mouse Tissue Slicing

Reflections on a decade of patching in adult brain slices

Using electrophysiological methods to examine e-cigarette flavors’ effect on dopamine neuron function

Studying Cell Motility in Glioblastoma with Precision-Cut Brain Sections

Using the Compresstome® in Immunotherapy Research

Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method

Genetic Manipulation of the Mouse Developing Hypothalamus Through In Utero Electroporation

Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

From two-dimensional to three-dimensional histopathology using a Compresstome®

Interactions between Saturated Fat, Cytokines, and Microglia in the Ventral Striatum

The locus coeruleus (LC) mediates behavioral flexibility

Investigating how sodium channels regulate the firing of cerebellar Purkinje neurons requires healthy brain slices

References

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