In-situ hybridization

Proper tissue fixture and preparation is crucial for getting the best in-situ hybridization (ISH) signals. Our Compresstome® and rotary microtomes allow you to cut thin sections down without having to freeze any tissue. 

In Situ MHC-tetramer Staining & Quantitative Analysis of Antigen-specific CD8 T Cells in Tissues

Fresh tissue can vary wildly in its level of difficulty to cut, due to a variety of factors like tissue type, and maturity of the animal (myelination). Often with other vibrating microtomes, they struggle to handle highly myelinated tissue or very soft neonatal tissue. The compression effect, along with multiple points of adjustment (Speed, oscillation, and agarose concentration) enables our instrument to better handle “difficult” to cut tissue. The Compresstome® isn’t just able to cut thinner than the competition, we believe that the evidence shows that we also provide higher quality cuts that preserve cell surface structures and help increase the number of healthy to dead cells. Researchers at University of Minnesota use a Compresstome® VF-300-0Z to section live tissue in their procedure to locate, quantify, and phenotype antigen-specific CD8 T cells.

Large specimen tissue sectioning

Fundamental research on HIV, the immune system, and neurological diseases have been done in non-human primates. The Compresstome® has been used to cut non-human primate lymphoid and genital tissues for immunohistochemistry (IHC) and in-situ hybridization (ISH) studies. For non-human primate brains, the Compresstome® has been crucial in helping cut thin (down to 40 µm) slices of fixed tissue. To help accommodate tissue samples of larger sizes from non-human primates, we have created the Compresstome® VF-800-0Z model, which can section entire primate brains as well as other organs.

References and Protocols

Microtomes from Precisionary Instruments have been used by labs around the world, and cited in hundreds of peer-reviewed publications. Explore these references by experiment, animal model, and organ system. For convenience, we also put together key experimental protocols to help you.