Animal: Mouse

Mouse Organotypic Brain Slices: A Platform to Investigate Remyelination Mechanisms in Frontotemporal Dementia

Sampada Tamhankar is a PhD candidate in the Department of Drug Discovery and Development at Auburn University, where she conducts her research in Dr. Rajesh Amin’s laboratory. Dr. Amin’s group focuses on developing novel small-molecule therapeutics for neurodegenerative diseases—including Alzheimer’s disease and frontotemporal dementia (FTD). Sampada’s work centers on understanding remyelination mechanisms in FTD using […]

Written by on December 9, 2025

Precision Cut Lung Slices: Basic Principles and Applications

Ken Chmiel (pronounced Shumel) is a Staff Research Associate at the University of California, Davis, with over 30 years of experience in mammalian cell culture. He first trained in the preparation of precision-cut lung slices (PCLS) at Harvard University in the lab of Dr. Xinbin Ai, in collaboration with Dr. Yan Bai. Since then, Ken […]

Written by on September 18, 2025

Causes and Consequences of Anterior Hypothalamic Area Activity

Dr. Brenton Laing leads a research lab at the University of Mississippi’s Department of BioMolecular Sciences, focusing on brain circuits that regulate survival behaviors, particularly those disrupted by stress and substance misuse. His work has revealed novel electrophysiological and molecular properties of anterior hypothalamic area (AHA) parvalbumin neurons, as well as their role in mediating behavioral responses to threats. Dr. Laing earned his PhD in bioenergetics from East Carolina University and completed his postdoctoral fellowship at the National Institute on Drug Abuse, where he trained under Dr. Yeka Aponte.

Written by on March 28, 2025

Genetic Manipulation of the Mouse Developing Hypothalamus Through In Utero Electroporation

Researchers have used the Compresstome® in their procedure to section mouse embryo hypothalamus that has been injected with DNA and electroporated. This procedure demonstrates how it is possible to transfect nuclei in the hypothalamus region which are less accessible than those in superficial regions. Following this procedure additional experiments can be performed such as immunohistochemistry and in situ hybridization.

Written by on April 25, 2023