Investigating how sodium channels regulate the firing of cerebellar Purkinje neurons requires healthy brain slices
Dr. Joseph Ransdell completed his undergraduate degree in Neuroscience at the University of Minnesota. He then…
Animal: Mouse
Genetic Manipulation of the Mouse Developing Hypothalamus Through In Utero Electroporation
Researchers have used the Compresstome® in their procedure to section mouse embryo hypothalamus that has been injected with DNA and electroporated. This procedure demonstrates how it is possible to transfect nuclei in the hypothalamus region which are less accessible than those in superficial regions. Following this procedure additional experiments can be performed such as immunohistochemistry and in situ hybridization.
Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method
Often heralded as leaders in the field, the Allen Brain institute performs pioneering research on all manner of brain tissue. Working with brain tissue can often be as frustrating as it is rewarding. Slicing brain tissue presents many challenges. The tissue is a combination of soft and fibrous regions. For over a decade, researchers at […]
Using calcium imaging and electrophysiology to explore the interaction between neurons and astrocytes in addiction
Dr. Junshi Wang is an enthusiastic neuroscientist who has been investigating neural mechanisms underlying drug addiction for…
Interactions between Saturated Fat, Cytokines, and Microglia in the Ventral Striatum
Dr. Fordahl will highlight how proinflammatory cytokines alter dopamine terminal function, and how increasing dietary fat…
Spatiotemporal Coordination of Stem Cell Behavior Following Alveolar Injury
In his PhD in Molecular Biology, working with Ben Hankamer at the Institute for Molecular Bioscience, University of…
Using the Compresstome® in Immunotherapy Research
Dr Astero Klampatsa (PhD) is a Team Leader in Cancer Immunotherapy at the Institute of Cancer Research, London,…
Using electrophysiological methods to examine e-cigarette flavors’ effect on dopamine neuron function
Dr. Henderson is an Assistant Professor in the Department of Biomedical Sciences at Marshall University’s Joan C. Edwards…
Reflections on a decade of patching in adult brain slices
Jonathan T. Ting is an Assistant Investigator at the Allen Institute, where he joined in 2013 to provide electrophysiology…
