Experiment: In-Situ Hybridization
Dr. Brenton Laing leads a research lab at the University of Mississippi’s Department of BioMolecular Sciences, focusing on brain circuits that regulate survival behaviors, particularly those disrupted by stress and substance misuse. His work has revealed novel electrophysiological and molecular properties of anterior hypothalamic area (AHA) parvalbumin neurons, as well as their role in mediating behavioral responses to threats. Dr. Laing earned his PhD in bioenergetics from East Carolina University and completed his postdoctoral fellowship at the National Institute on Drug Abuse, where he trained under Dr. Yeka Aponte.
Written by clark on March 28, 2025
Researchers have used the Compresstome® in their procedure to section mouse embryo hypothalamus that has been injected with DNA and electroporated. This procedure demonstrates how it is possible to transfect nuclei in the hypothalamus region which are less accessible than those in superficial regions. Following this procedure additional experiments can be performed such as immunohistochemistry and in situ hybridization.
Written by Admin on April 25, 2023
Explore how scientists use the Compresstome® vibrating microtome to create tissue slices that combine lipophilic dye tracing, whole mount in situ hybridization, immunohistochemistry, and histology to extract the maximal possible amount of data.
Written by Admin on April 23, 2023
