Genetic Sequencing (Single-Cell Isolation)

In research, single-cell isolation and genetic sequencing are powerful techniques for unraveling the complexities of biological systems at the cellular level. The success of these studies critically depends on obtaining healthy and precise tissue slices. High-quality tissue samples ensure that each isolated cell represents an accurate snapshot of the organism’s biology, minimizing contamination and preserving the integrity of genetic material. This precision is paramount in unlocking insights into cellular diversity, gene expression patterns, and genetic mutations, ultimately advancing our understanding of complex diseases, developmental processes, and the intricate machinery of life itself.

*Patch-Seq diagram illustrating the three main data types we obtain from the technique: Electrophysiology Properties, Transcriptome Analysis and Morphological reconstruction.*

Recommended Models

VF-510-0Z

Compresstome vibrating microtome

Learn more

Not sure which model is right for your needs?

Real lab examples

References

Hodge RD, Bakken TE, Miller JA, Smith KA, Barkan ER, Graybuck LT, Close JL, Long B, Johansen N, Penn O, Yao Z, Eggermont J, Höllt T, Levi BP, Shehata SI, Aevermann B, Beller A, Bertagnolli D, Brouner K, Casper T, Cobbs C, Dalley R, Dee N, Ding SL, Ellenbogen RG, Fong O, Garren E, Goldy J, Gwinn RP, Hirschstein D, Keene CD, Keshk M, Ko AL, Lathia K, Mahfouz A, Maltzer Z, McGraw M, Nguyen TN, Nyhus J, Ojemann JG, Oldre A, Parry S, Reynolds S, Rimorin C, Shapovalova NV, Somasundaram S, Szafer A, Thomsen ER, Tieu M, Quon G, Scheuermann RH, Yuste R, Sunkin SM, Lelieveldt B, Feng D, Ng L, Bernard A, Hawrylycz M, Phillips JW, Tasic B, Zeng H, Jones AR, Koch C, Lein ES.Conserved cell types with divergent features in human versus mouse cortex. Nature. 2019 Sep;573(7772):61-68. Epub 2019 Aug 21. PMID: 31435019; PMCID: PMC6919571. Download PDF

Kim EJ, Zhang Z, Huang L, Ito-Cole T, Jacobs MW, Juavinett AL, Senturk G, Hu M, Ku M, Ecker JR, Callaway EM. Extraction of Distinct Neuronal Cell Types from within a Genetically Continuous Population. Neuron. 2020 Jul 22;107(2):274-282.e6. Epub 2020 May 11. PMID: 32396852; PMCID: PMC7381365. Download PDF

Tasic B, Yao Z, Graybuck LT, Smith KA, Nguyen TN, Bertagnolli D, Goldy J, Garren E, Economo MN, Viswanathan S, Penn O, Bakken T, Menon V, Miller J, Fong O, Hirokawa KE, Lathia K, Rimorin C, Tieu M, Larsen R, Casper T, Barkan E, Kroll M, Parry S, Shapovalova NV, Hirschstein D, Pendergraft J, Sullivan HA, Kim TK, Szafer A, Dee N, Groblewski P, Wickersham I, Cetin A, Harris JA, Levi BP, Sunkin SM, Madisen L, Daigle TL, Looger L, Bernard A, Phillips J, Lein E, Hawrylycz M, Svoboda K, Jones AR, Koch C, Zeng H. Shared and distinct transcriptomic cell types across neocortical areas. Nature. 2018 Nov;563(7729):72-78. Epub 2018 Oct 31. PMID: 30382198; PMCID: PMC6456269. Download PDF