Webinars

Precisionary Instruments offers free scientific webinars by experts in their academic field

Precisionary Instruments Webinars

Would you like to find out more about how our Compresstome® vibrating microtomes and rotary microtomes are used by actual scientists in their research? Are you looking for vibratome cutting tips? Explore our webinars! We invited academic experts from a wide spectrum of topics to share how they use our microtomes in their labs. Application topics covered include electrophysiology, immunotherapy, immunohistochemistry, and much more.

Would you like to present a webinar with us?

We welcome you to present your research and findings with Precisionary Instruments. We take care of all hosting and logistical planning.

Joseph L. Ransdell, PHD

Assistant Professor, Department of Biology

 

Miami University

Oxford, OH

Investigating how sodium channels regulate the firing of cerebellar Purkinje neurons requires healthy brain slices

Original webinar date: November 15, 2022

 

Dr. Joseph Ransdell completed his undergraduate degree in Neuroscience at the University of Minnesota. He then completed his Ph.D. at the University of Missouri Columbia and studied the regulation of membrane excitability in crab motor neurons. After receiving his Ph.D., he joined the lab of Jeanne Nerbonne at Washington University. There, he continued using electrophysiological methods to test how ion channel accessory proteins regulate the excitability of various types of mouse central neurons. In 2020, Dr. Ransdell started his independent lab at Miami University in Oxford, OH. He continues to work with mouse models and his team has centered their focus on the regulation of voltage-gated sodium channels and the contributions of the channels to the functioning of cerebellar circuits during health and disease.

In this webinar, Dr. Ransdell explores how the Compresstome vibrating microtome is used to produce healthy brain slices for electrophysiology. He studies adult Purkinje neurons in mouse cerebellar brain slices.

KATERINA TSILINGIRI, PHD

Biomedical Research Foundation

Academy of Athens (BRFAA)

Greece

Slicing up the tumor: Lessons from attempted lung tumor slice cultures

Original webinar date: September 20, 2022

 

Dr. Tsilingiri was born and raised in Athens, Greece. She studied Biochemistry and Biotechnology in Thessaly and then completed research training in Spain, Italy and Scotland. Her main expertise is on basic immunology and aspects of the interplay between nutrition and the immune system. She has extensive experience in explant slice cultures. 

 

Currently, she is working on tumor immunotherapy and using the Compresstome vibrating microtome to examine the interaction between tumor tissues and autologous lymph node cells in slice cultures. This work is being carried out in the frame of an EU-funded Consortium, Tumour-LNoC (Tumour-Lymph node on a chip), with the ultimate goal of mimicking the metastatic process on a chip and monitor metastasizing cells in real time. 

 

In this webinar, Dr. Tsilingiri explores how she uses the Compresstome vibrating microtome to make healthy tumor slices in her research of lung cancer.

TERENCE WONG, PHD

Assistant Professor, Department of Chemical and Biological Engineering (CBE)

 

Director, Research Center for Medical Imaging and Analysis (CMIA)

 

Hong Kong University of Science and Technology (HKUST)

From two-dimensional to three-dimensional histopathology using a Compresstome®

Original webinar date: July 12, 2022

Terence Tsz Wai Wong received his B.Eng. and M.Phil. degrees both from the University of Hong Kong in 2011 and 2013, respectively. He studied Biomedical Engineering at Washington University in St. Louis (WUSTL) and Medical Engineering at California Institute of Technology (Caltech), under the tutelage of Prof. Lihong V. Wang (member of the National Academy of Engineering and Inventors) for his Ph.D. degree. Right after his Ph.D. graduation, he joined the Hong Kong University of Science and Technology (HKUST) as an Assistant Professor in the Department of Chemical and Biological Engineering (CBE). He is also the Director of the HKUST Research Center for Medical Imaging and Analysis (CMIA). With the integration of optical/photoacoustic imaging and deep-learning algorithms, his research focuses on developing smart optical and photoacoustic devices to enable label-free and high-speed histological imaging, three-dimensional whole-organ imaging, and low-cost cancer-targeting deep-tissue imaging. He is an author or co-author of over 50 publications in top peer-reviewed journals (including Nature Photonics, Nature Methods, Nature Communications, Science Advances, Advanced Science, etc), conference papers, and book chapters, and has five U.S. patents.

In this webinar, Dr. Wong shares how he built a custom-made Compresstome® for high-speed histological 3D imaging of whole organs like brains.

ASTERO KLAMPATSA, PHD

Team Leader in Cancer Immunotherapy

 

Institute of Cancer Research, London

Using the Compresstome® in Immunotherapy Research

Original webinar date: May 23, 2022

Dr Astero Klampatsa (PhD) is a Team Leader in Cancer Immunotherapy at the Institute of Cancer Research, London, UK and a Senior Lecturer in King’s College London, UK. She was awarded a PhD in Research Oncology (focused in mesothelioma apoptotic and hypoxia pathways) from Queen Mary’s College, University of London, UK. As a postdoctoral fellow, she gained expertise in CAR T cell immunotherapy and immunobiology of thoracic cancers at King’s College London, UK, and at the University of Pennsylvania, USA. Since 2019, Dr Klampatsa has been leading her own team, the Thoracic Oncology Immunotherapy Group, focusing on developing novel CAR T cell therapies for mesothelioma and lung cancer, as well as the immunobiology of these malignancies for identification of markers of response to immunotherapy.

In this webinar, Dr. Klampatsa will discuss how the Compresstome® was used to create precision-cut tumor slices (PCTS) as an ex vivo model for immunotherapy research.

YIYING ZHANG, MD-PHD

Assistant Professor of Anesthesia

 

Massachusetts General Hospital

 

Harvard Medical School

“3D” use of animal tissues in experimental design

Original webinar date: March 1, 2022

 

Have you wondered how one mouse brain may be used for multiple experiments? Come discover the strategy behind using animal tissues for multi-use research experiments, so that your tissue samples can go further.

 

Dr. Yiying Zhang from Harvard Medical School and Massachusetts General Hospital is our guest webinar speaker.

 

Dr. Yiying Zhang has been studying neuropathogenesis, anesthesia neurotoxicity, anesthesia/surgery toxicity, postoperative neurocognitive disorder and postoperative delirium (POD) in Alzheimer’s disease (AD) since 2009. Specifically, she has studied the effects of anesthetics on mitochondrial function in vitro and in vivo, as well as the potential association between neuroinflammation and mitochondrial function following the anesthesia and/or surgery. Recently, Dr. Zhang has focused on studying potential gut microbiota-neuroinflammation-mitochondria cascade in the pathogenesis of POD. For this Precisionary webinar, Dr. Zhang will discuss a “3D” use of animal tissues in planning experimental designs in academic research.

IAN MACCLOUD

Director of Operations

GRIFFIN FERRARA

Business Development Lead

Making precision-cut tissue slices for ex vivo assay services

Original webinar date: September 14, 2021

 

Have you wondered about how to make precision-cut tissue slices and their experimental applications? Precisionary Instruments has worked with Visikol, a contract research services company focused on leveraging advanced imaging, 3D cell culture assays and digital pathology to accelerate the drug discovery and development process. 

 

In this webinar, Visikol experts explain the need for in vitro liver models to study livery injury. Our guest speakers demonstrate the standard assay format for creating precision-cut liver slices (PCLS), explain how the Compresstome® VF-310-0Z vibrating microtome helps create uniform tissue slices that can be meaningfully compared between treatments, go through how to use the Compresstome® step-by-step for making PCLS, and discuss how slices are made from normal human liver tissue, diseased human liver tissue, and mouse model liver.

BRANDON HENDERSON, PHD

Assistant Professor Joan C. Edwards School of Medicine at Marshall University

Using electrophysiological methods to examine e-cigarette flavors’ effect on dopamine neuron function

Original webinar date: July 6, 2021


Dr. Henderson is an Assistant Professor in the Department of Biomedical Sciences at Marshall University’s Joan C. Edwards School of Medicine. He received a BS (with distinction) in Chemistry at The Ohio State University, where he started research, initially as an Analytical Chemist in the lab of Dr. John Olesik. He went on to receive his PhD in Pharmacology at The Ohio State University in the lab of Dr. Dennis McKay. Afterwards, his professional journey continued his development as a NIDA-NIH Postdoctoral Fellow at the California Institute of Technology in the lab of Dr. Henry Lester. Before starting his faculty position at Marshall University, Dr. Henderson also trained as a visiting postdoctoral fellow at Yale University (under Dr Nii Addy and Dr. Marina Picciotto).


In addition to his responsibilities at Marshall University, Dr. Henderson is now one of two co-Chairs for the Basic Science Network in the Society for Research on Nicotine and Tobacco (www.SRNT.org).


The Henderson lab focuses on the role tobacco and vaping flavors play in addiction-related behaviors. Thus far, we have shown that menthol and green apple flavors can enhance nicotine vapor self-administration and do so by directly altering dopamine neurons in the midbrain.

JONATHAN TING, PHD

Associate Investigator Allen Institute

Reflections on a decade of patching in adult brain slices

Original webinar date: March 22, 2021

Jonathan T. Ting is an Assistant Investigator at the Allen Institute, where he joined in 2013 to provide electrophysiology expertise for the Human Cell Types program, and to develop functional assays on human ex vivo brain slides. Dr. Ting has more than 15 years of experience in patch clamp electrophysiology. He studied the neural circuitry basis of psychiatric disorders during his postdoctoral fellowships at Duke University and the McGovern Institute for Brain Research at MIT, and he developed and characterized several transgenic mouse lines now widely employed for nervous system research involving optogenetics.

For this webinar, Dr. Ting provides reflections of his experience on a decade of patching adult brain slices. He will:

    • Discuss which key steps in the brain slice process is most important and why
    • Challenge our conventional beliefs of slicing solutions and methodologies
    • Recommend tips and tricks based on his experience and research