Which Buffer Solution Should You Use? A Guide for Tissue Sectioning Applications
Formulas and rationale for live sectioning of brain, heart, lung, liver, organoids, and tumors There is no universal buffer. Start from six requirements every sectioning buffer shares (isotonic ~300 mOsm, pH 7.3 to 7.4, balanced ions, glucose, oxygenation, ice-cold), then add tissue-specific protectants. Brain: carbogen-bubbled aCSF; switch to an NMDG- or sucrose-substituted aCSF for cutting […]