Real Lab Example Archives

Experiment: Real Lab Example

In Situ MHC-tetramer Staining & Quantitative Analysis of Antigen-specific CD8 T Cells in Tissues

Fresh tissue can vary wildly in its level of difficulty to cut, due to a variety of factors like tissue type, and maturity of the animal (myelination). Often with other vibrating microtomes, they struggle to handle highly myelinated tissue or very soft neonatal tissue. The compression effect, along with multiple points of adjustment (speed, oscillation, […]

Written by Admin on April 25, 2023

Immunology and Infection: The Compresstome® for Precision-Cut Lung Slices

The Compresstome® has been widely used by researchers worldwide for making precision-cut lung slices (PCLS). The Compresstome® uses agarose embedding prior to slicing to allow for the preservation of open alveoli and better tissue compliance. The video above shows Compresstome® sectioning PCLS for immunostaining to visualize the localization of various immune cell types in the lung. This protocol can be extended […]

Written by Admin on April 25, 2023

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Experiment: Real Lab Example

In Situ MHC-tetramer Staining & Quantitative Analysis of Antigen-specific CD8 T Cells in Tissues

Immunology and Infection: The Compresstome® for Precision-Cut Lung Slices

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